{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T01:24:58Z","timestamp":1771896298959,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,7,27]],"date-time":"2015-07-27T00:00:00Z","timestamp":1437955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>The regeneration of nerves of the peripheral nervous system after injuries is a complex process. This study presents a novel in vitro neurite regeneration concept to investigate the regeneration of neurons and their processes with different concentrations of neurotrophic factors. The core part of the concept is a transparent microfluidic neurite isolation (NI) device affixed on top of a microelectrode array (MEA), providing a fast and easy way to assess both the growth and the electrical activity of neurites. The NI\u2010MEA isolates neurites from the culture with microchannels that serve as guidance tubes, equipped with microelectrodes. Thus, the NI\u2010MEA allows neurite growth, as observed by microscopy, to be correlated with neurite electrical activity, as measured by electrophysiological recordings. To demonstrate proof of concept of neurite regeneration, we cultured cells from the superior cervical ganglion of postnatal mice under different concentrations of nerve growth factor (NGF). During the regeneration process, we observed an increase in the number of neurites entering the microchannels along with an increase in spike activity recorded by the microelectrodes in the microchannels. We also observed a concentration\u2010dependent effect of neurotrophic factor on the excitability of the growing neurites, with neurites bathed in 20 ng\/ml NGF exhibiting enhanced early growth. Thus, our neurite regeneration concept with the NI\u2010MEA device allows further study of neurotrophic factors and reduces the requirement for in vivo experiments on the regeneration of peripheral nerves after injury. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23598","type":"journal-article","created":{"date-parts":[[2015,7,27]],"date-time":"2015-07-27T14:36:22Z","timestamp":1438007782000},"page":"1631-1640","source":"Crossref","is-referenced-by-count":10,"title":["Investigation of neurotrophic factor concentrations with a novel in vitro concept for peripheral nerve regeneration"],"prefix":"10.1002","volume":"93","author":[{"given":"Johann K.","family":"Mika","sequence":"first","affiliation":[{"name":"Institute for Solid State Electronics, Vienna University of Technology Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Department of Pathobiology of the Nervous System (Center for Brain Research) Medical University of Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinz D.","family":"Wanzenboeck","sequence":"additional","affiliation":[{"name":"Institute for Solid State Electronics, Vienna University of Technology Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Scholze","sequence":"additional","affiliation":[{"name":"Department of Pathobiology of the Nervous System (Center for Brain Research) Medical University of Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmerich","family":"Bertagnolli","sequence":"additional","affiliation":[{"name":"Institute for Solid State Electronics, Vienna University of Technology Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2008.06.005"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.4149\/gpb_2012_016"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-009-0009-x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-013-9474-z"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(95)00235-B"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1389-5"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1964"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-05-01642.1997"},{"key":"e_1_2_8_10_1","first-page":"211","article-title":"A neural tissue interfacing chip for in\u2010vitro applications with 32k recording\/stimulation channels on an active area of 2.6 mm2","volume":"41","author":"Eversmann B","year":"2011","journal-title":"Proc ESSDIRC"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.075788"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2008.11.028"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02740621"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20778"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1054\/jhsb.2000.0497"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1952.sp004764"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311-011-0072-y"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20761"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4886(03)00258-9"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00542-007-0417-y"},{"key":"e_1_2_8_21_1","first-page":"1","article-title":"Electrical and morphological properties of neurites investigated by a novel microelectrode\u2013microfluidic device","author":"Mika JK","year":"2013","journal-title":"Intrinsic Activity"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/scientificamerican0392-44"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0996-976"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.24.1.1217"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2012.265"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1529-8027.2002.02002.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth777"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(72)90481-8"},{"key":"e_1_2_8_29_1","first-page":"165","volume-title":"International review of neurobiology","author":"Tos P","year":"2013"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20635"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2014.02.065"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.23598","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.23598","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T12:36:42Z","timestamp":1696336602000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.23598"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,27]]},"references-count":30,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1002\/jnr.23598"],"URL":"https:\/\/doi.org\/10.1002\/jnr.23598","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,27]]}}}