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Diabetic neuropathy is often associated with spontaneous pain and altered thermal perception. This study assesses effects of MG on TRPM8, an ion channel involved in innocuous cold sensing and cold allodynia and also in cold\u2010mediated analgesia. Acute treatment with MG inhibited the activation of recombinant rat and human transient receptor potential melastatin type 8 (TRPM8) by cold and chemical agonists. A similar effect was observed when native TRPM8 was investigated in cultured rat dorsal root ganglion (DRG) neurons. DRG neurons treated with MG for 16\u201324\u2009hr displayed a significant reduction in the fraction of cold\u2010 and menthol\u2010sensitive neurons, most likely expressing TRPM8. The fraction of allyl isothiocyanate\u2010sensitive neurons was also reduced, and the coexpression among different neuronal populations was affected. The same prolonged exposure to MG significantly reduced the expression of TRPM8 at the mRNA level. Overall, our data provide evidence for decreased activity and expression level of TRPM8 in the presence of MG, which may be linked to some of the alterations in pain and temperature sensing reported by diabetic patients. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23700","type":"journal-article","created":{"date-parts":[[2015,12,2]],"date-time":"2015-12-02T13:42:56Z","timestamp":1449063776000},"page":"282-294","source":"Crossref","is-referenced-by-count":10,"title":["Glycolytic metabolite methylglyoxal inhibits cold and menthol activation of the transient receptor potential melastatin type 8 channel"],"prefix":"10.1002","volume":"94","author":[{"given":"A.C.","family":"Ciobanu","sequence":"first","affiliation":[{"name":"Department of Anatomy Physiology, and Biophysics, Faculty of Biology, University of Bucharest  Bucharest 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