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This study uses peripheral nerve grafts (PNGs) as scaffolds for axonal growth while delivering neurotrophin\u20103 (NT\u20103) via calcium phosphate (CaP) coatings on surgical sutures. CaP coating was grown on sutures, and NT\u20103 binding and release were characterized in vitro. Then, the NT\u20103\u2010loaded sutures were tested in a complete SCI model. Rats were analyzed for functional improvement and axonal growth into the grafts. The CaP\u2010coated sutures exhibited a burst release of NT\u20103, followed by a sustained release for at least 20 days. Functionally, the rats with PNGs\u2009+\u2009NT\u20103\u2010loaded sutures and the rats treated with PNGs scored significantly higher than controls on day 56 postoperatively. However, functional scores in rats treated with PNGs\u2009+\u2009NT\u20103\u2010loaded suture were not significantly different from those of rats treated with PNGs alone. Cholera toxin subunit B (CTB) labeling rostral to the graft was not observed in any controls, but CTB labeling rostral to the graft was observed in almost all rats that had had a PNG. Neurofilament labeling on transverse sections of the graft revealed that the rats treated with the NT\u20103\u2010loaded sutures had significantly more axons per graft than rats treated with an NT\u20103 injection and rats without NT\u20103. These data demonstrate that PNGs serve as scaffolds for axonal growth after SCI and that CaP\u2010coated sutures can efficiently release NT\u20103 to increase axonal regeneration. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23730","type":"journal-article","created":{"date-parts":[[2016,3,26]],"date-time":"2016-03-26T14:04:26Z","timestamp":1459001066000},"page":"645-652","source":"Crossref","is-referenced-by-count":20,"title":["Sustained release of neurotrophin\u20103 via calcium phosphate\u2010coated sutures promotes axonal regeneration after spinal cord injury"],"prefix":"10.1002","volume":"94","author":[{"given":"Amgad","family":"Hanna","sequence":"first","affiliation":[{"name":"Department of Neurological Surgery University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel L.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery University of Wisconsin Madison 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