{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T15:41:47Z","timestamp":1790264507868,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,10,11]],"date-time":"2016-10-11T00:00:00Z","timestamp":1476144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"crossref","award":["P304\/12\/G069"],"award-info":[{"award-number":["P304\/12\/G069"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]},{"name":"European Regional Development Fund - Project FNUSA-ICRC","award":["CZ.1.05\/1.1.00\/02.0123"],"award-info":[{"award-number":["CZ.1.05\/1.1.00\/02.0123"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:p>Diffusion\u2010weighted magnetic resonance (DW\u2010MR) is an important diagnostic tool in Huntington disease (HD), a fatal hereditary neurodegenerative disorder. To clarify the nature of diffusivity changes in HD, we compared the apparent diffusion coefficient of water (ADC<jats:sub>W<\/jats:sub>) acquired by DW\u2010MR with extracellular space volume fraction \u03b1 and tortuosity \u03bb, measured by the iontophoretic method in the R6\/2 mouse model of HD and in wild\u2010type controls (WT). In anisotropic globus pallidus (GP), diffusion measurements were performed in the mediolateral (<jats:italic>x<\/jats:italic>), rostrocaudal (<jats:italic>y<\/jats:italic>), and ventrodorsal (<jats:italic>z<\/jats:italic>) axes. In HD animals, we detected an increase in ADC<jats:sub>W<\/jats:sub> in all axes and larger \u03b1 than in WT mice. No significant difference between WT and HD mice was found in the values of tortuosity (\u03bb<jats:sub>x<\/jats:sub>, \u03bb<jats:sub>y<\/jats:sub>, \u03bb<jats:sub>z<\/jats:sub>). Despite structural changes in GP, diffusion anisotropy was unaffected in HD mice. Immunohistochemical analysis revealed in HD mice weaker expression of extracellular matrix and a decrease in neuron numbers compared with WT mice. Glial fibrillary acidic protein staining detected astrogliosis\u2010like changes in the morphology of astrocytic processes in HD GP. In the somatosensory cortex, no significant differences in the studied parameters were found. We conclude that in the R6\/2 model of HD, a decrease in the number of neurons in the GP results in increased ADC<jats:sub>W<\/jats:sub> and \u03b1 values. Values of \u03bb were not significantly changed as the increase of diffusion obstacles formed by reactive astrocytes was compensated for by the extracellular matrix reduction. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23965","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T14:25:42Z","timestamp":1476368742000},"page":"1474-1484","source":"Crossref","is-referenced-by-count":11,"title":["Brain Diffusivity and Structural Changes in the R6\/2 Mouse Model of Huntington Disease"],"prefix":"10.1002","volume":"95","author":[{"given":"Ivan","family":"Vorisek","sequence":"first","affiliation":[{"name":"Department of Neuroscience Institute of Experimental Medicine AS CR, v.v.i. Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Syka","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Institute of Experimental Medicine AS CR, v.v.i. 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