{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:00:45Z","timestamp":1790820045543,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T00:00:00Z","timestamp":1480377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81271363"],"award-info":[{"award-number":["81271363"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571194"],"award-info":[{"award-number":["81571194"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aims to explore the temporal changes of cytotoxic CD8<jats:sup>+<\/jats:sup>CD28<jats:sup>+<\/jats:sup> and regulatory CD8<jats:sup>+<\/jats:sup> CD28<jats:sup>\u2212<\/jats:sup> T\u2010cell subsets in the lesion microenvironment after spinal cord injury (SCI) in rats, by combination of immunohistochemistry (IHC) and flow cytometry (FCM). In the sham\u2010opened spinal cord, few CD8<jats:sup>+<\/jats:sup> T cells were found. After SCI, the CD8<jats:sup>+<\/jats:sup> T cells were detected at one day post\u2010injury (dpi), then markedly increased and were significantly higher at 3, 7, and 14 dpi compared with one dpi (p\u2009&lt;\u20090.01), the highest being seven dpi. In CD8<jats:sup>+<\/jats:sup> T cells, more than 90% were CD28<jats:sup>+<\/jats:sup>, and there were only small part of CD28<jats:sup>\u2212<\/jats:sup> (\u2009&lt;\u200910%). After 14 days, the infiltrated CD8<jats:sup>+<\/jats:sup> T cells were significantly decreased, and few could be found in good condition at 21 and 28 dpi. Annexin V and propidium iodide (PI) staining showed that the percentages of apoptotic\/necrotic CD8<jats:sup>+<\/jats:sup> cells at 14 dpi and 21 dpi were significantly higher than those of the other early time\u2010points (p\u2009&lt;\u20090.01). These results indicate that CD8<jats:sup>+<\/jats:sup> T cells could rapidly infiltrate into the injured spinal cords and survive two weeks, however, cytotoxic CD8<jats:sup>+<\/jats:sup> T cells were dominant. Therefore, two weeks after injury might be the \u201ctime window\u201d for treating SCI by prolonging survival times and increasing the fraction of CD8<jats:sup>+<\/jats:sup> regulatory T\u2010cells. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.23993","type":"journal-article","created":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T15:07:02Z","timestamp":1480432022000},"page":"1666-1676","source":"Crossref","is-referenced-by-count":25,"title":["Temporal kinetics of CD8<sup>+<\/sup>CD28<sup>+<\/sup> and CD8<sup>+<\/sup>CD28<sup>\u2212<\/sup> T lymphocytes in the injured rat spinal cord"],"prefix":"10.1002","volume":"95","author":[{"given":"Yan","family":"Wu","sequence":"first","affiliation":[{"name":"Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Department of Immunology Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College Anhui 233030 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Hong","family":"Lin","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Department of Immunology Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College Anhui 233030 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling\u2010Ling","family":"Shi","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Department of Immunology Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College Anhui 233030 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zong\u2010Feng","family":"Yao","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu\u2010Mei","family":"Xie","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng\u2010Song","family":"Jiang","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Department of Immunology Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College Anhui 233030 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Tang","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Department of Immunology Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College Anhui 233030 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Guo","family":"Hu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He\u2010Zuo","family":"L\u00fc","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Anhui Key Laboratory of Tissue Transplantation, the First Affiliated Hospital of Bengbu Medical College Anhui 233004 P.R. China"},{"name":"Department of Immunology Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College Anhui 233030 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awp322"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.10578"},{"issue":"3","key":"e_1_2_10_4_1","first-page":"169","article-title":"CD28 costimulatory molecule\u2010\u2010expression, structure and function","volume":"50","author":"Bocko D","year":"2002","journal-title":"Arch Immunol Ther Exp (Warsz)"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.115.010592"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2014.3429"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1789.2008.00980.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23612"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22348"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2015.08.064"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2009.06.013"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012478"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2007.06.009"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.04046.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.7.4323"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.05.019"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2016.01.002"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneurol.2010.312"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.232565399"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1157\/13095870"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2008.06.006"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2014.11.007"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3171\/spi.2006.4.3.233"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2014.01.025"},{"issue":"50","key":"e_1_2_10_25_1","first-page":"2597","article-title":"Modified annexin V\/propidium iodide apoptosis assay for accurate assessment of cell death","author":"Rieger AM","year":"2011","journal-title":"J Vis Exp"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1997.tb01450.x"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0124923"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2011.03470.x"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2008.08.283"},{"key":"e_1_2_10_30_1","first-page":"9 49 41","article-title":"Measurement and Characterization of Apoptosis by Flow Cytometry","volume":"77","author":"Telford W","year":"2016","journal-title":"Curr Protoc Cytom"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19980101)31:1<1::AID-CYTO1>3.0.CO;2-R"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(95)00072-I"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(02)00367-3"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/sji.12276"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/08977150360547116"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-11-65"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.23993","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.23993","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.23993","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T18:57:03Z","timestamp":1696532223000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.23993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,29]]},"references-count":35,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1002\/jnr.23993"],"URL":"https:\/\/doi.org\/10.1002\/jnr.23993","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,29]]}}}