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However, specific gestational timing when developing organs are most sensitive to alcohol exposure is unclear. In this study, we examined the temporal effects of embryonic alcohol exposure on octavolateral organs in zebrafish (<jats:italic>Danio rerio<\/jats:italic>), including inner ears and lateral line neuromasts that function in hearing, balance, and hydrodynamic detection, respectively. To determine an alcohol\u2010sensitive period in the first 24 hours post fertilization (hpf), Et(krt4:EGFP)<jats:sup>sqet4<\/jats:sup>zebrafish that express green fluorescent protein in sensory hair cells were treated in 2% alcohol for 2, 3, and 5\u2010hours. Octavolateral organs of control and alcohol\u2010exposed larvae were examined at 3, 5, and 7 days post fertilization (dpf). Using confocal and light microscopy, we found that alcohol\u2010exposed larvae had significantly smaller otic vesicles and saccular otoliths than control larvae at 3 dpf. Only alcohol\u2010exposed larvae from 12\u201017 hpf had smaller otic vesicles at 5 dpf, smaller saccular otoliths at 7 dpf and fewer saccular hair cells, neuromasts and hair cells per neuromast at 3 dpf. In addition, auditory function was assessed by microphonic potential recordings from inner ear hair cells in response to 200\u2010Hz stimulation. Hearing sensitivity was reduced for alcohol\u2010exposed larvae from 7\u201012 and 12\u201017 hpf. Our results show that 12\u201017 hpf is an alcohol\u2010sensitive time window when morphology and function of zebrafish octavolateral organs are most vulnerable to alcohol exposure. This study implies that embryonic alcohol exposure timing during early development can influence severity of hearing deficits. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.24017","type":"journal-article","created":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T12:00:54Z","timestamp":1484913654000},"page":"1194-1203","source":"Crossref","is-referenced-by-count":4,"title":["The alcohol\u2010sensitive period during early octavolateral organ development in zebrafish (<i>Danio rerio<\/i>)"],"prefix":"10.1002","volume":"95","author":[{"given":"Lilliann Y.","family":"Zamora","sequence":"first","affiliation":[{"name":"University of Miami, Department of Biology Coral Gables Florida"},{"name":"University of Miami, Neuroscience Program Miami Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayla C.","family":"Miguel","sequence":"additional","affiliation":[{"name":"University of Miami, Neuroscience Program Miami 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