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Moreover, they are likely to disrupt the metabolic support of astrocytes to neurons, as well as the uptake of nutrients from circulation. By employing<jats:sup>13<\/jats:sup>C magnetic resonance spectroscopy (MRS)<jats:italic>in vivo<\/jats:italic>at high magnetic field, we characterized neuronal and astrocytic pathways of energy metabolism in the rat cortex under thiopental anaesthesia. The neuronal tricarboxylic acid (TCA) cycle rate was 0.46\u2009\u00b1\u20090.02\u2009\u00b5mol\/g\/min, and the rate of the glutamate\u2010glutamine cycle was 0.09\u2009\u00b1\u20090.02\u2009\u00b5mol\/g\/min. In astrocytes, the TCA cycle rate was 0.16\u2009\u00b1\u20090.02\u2009\u00b5mol\/g\/min, accounting for a quarter of whole brain glucose oxidation, pyruvate carboxylase rate was 0.02\u2009\u00b1\u20090.01\u2009\u00b5mol\/g\/min, and glutamine synthetase was 0.12\u2009\u00b1\u20090.01\u2009\u00b5mol\/g\/min. Relative to previous experiments under light \u03b1\u2010chloralose anaesthesia, thiopental reduced oxidative metabolism in neurons and even more so in astrocytes. Interestingly, total oxidative metabolism in the cortex under thiopental anaesthesia surpassed the rate of pyruvate production by glycolysis, indicating substantial utilisation of substrates other than glucose, likely plasma lactate. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jnr.24032","type":"journal-article","created":{"date-parts":[[2017,3,19]],"date-time":"2017-03-19T14:16:39Z","timestamp":1489932999000},"page":"2297-2306","source":"Crossref","is-referenced-by-count":20,"title":["Energy metabolism in the rat cortex under thiopental anaesthesia measured<i>In Vivo<\/i>by<sup>13<\/sup>C MRS"],"prefix":"10.1002","volume":"95","author":[{"given":"Sarah","family":"Sonnay","sequence":"first","affiliation":[{"name":"Laboratory for Functional and Metabolic Imaging, \u00c9cole Polytechnique F\u00e9d\u00e9rale Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5984-1574","authenticated-orcid":false,"given":"Jo\u00e3o M. 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