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Here we have expanded and refined the versatility of luminopsin tools by fusing an alternative luciferase variant with high light emission, <jats:italic>Gaussia<\/jats:italic> luciferase mutant GLucM23, to depolarizing and hyperpolarizing channelrhodopsins with increased light sensitivity. The combination of GLucM23 with <jats:italic>Volvox<\/jats:italic> channelrhodopsin\u20101 produced LMO4, while combining GLucM23 with the anion channelrhodopsin iChloC yielded iLMO4. We found efficient activation of these channelrhodopsins in the presence of the luciferase substrate, as indicated by responses measured in both single neurons and in neuronal populations of mice and rats, as well as by changes in male rat behavior during amphetamine\u2010induced rotations. 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