{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:31:00Z","timestamp":1790033460998,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,11,7]],"date-time":"2017-11-07T00:00:00Z","timestamp":1510012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of human induced pluripotent stem cells (hiPSCs) eliminates the ethical issues associated with fetal or embryonic materials, thus allowing progress in cell therapy research for ischemic stroke. Strict regulation of cell therapy development requires the xeno\u2010free condition to eliminate clinical complications. Maintenance of hiPSCs with feeder\u2010free condition presents a higher degree of spontaneous differentiation in comparison with conventional cultures. Therefore, feeder\u2010free derivation might be not ideal for developing transplantable hiPSC derivatives. We developed the feeder\u2010free condition for differentiation of cortical neurons from hiPSCs. Then, we evaluated the cells' characteristics upon transplantation into the sham and focal brain ischemia on adult male Wistar rats. Grafts in lesioned brains demonstrated polarized reactivity toward the ischemic border, indicated by directional preferences in axonal outgrowth and cellular migration, with no influence on graft survival. Following the transplantation, forelimb asymmetry was better restored compared with controls. Herein, we provide evidence to support the use of the xeno\u2010free condition for the development of cell therapy for ischemic stroke.<\/jats:p>","DOI":"10.1002\/jnr.24197","type":"journal-article","created":{"date-parts":[[2017,11,7]],"date-time":"2017-11-07T07:08:44Z","timestamp":1510038524000},"page":"863-874","source":"Crossref","is-referenced-by-count":13,"title":["Transplantation of feeder\u2010free human induced pluripotent stem cell\u2013derived cortical neuron progenitors in adult male Wistar rats with focal brain ischemia"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2937-8130","authenticated-orcid":false,"given":"Yulius","family":"Hermanto","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Graduate School of Medicine, Kyoto University Kyoto Japan"},{"name":"Department of Clinical Application, Center of iPS Cell Research and Application Kyoto University Kyoto Japan"},{"name":"Department of Neurosurgery, Faculty of Medicine Universitas Padjadjaran Bandung Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Sunohara","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Graduate School of Medicine, Kyoto University Kyoto Japan"},{"name":"Department of Clinical Application, Center of iPS Cell Research and Application Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Faried","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Faculty of Medicine Universitas Padjadjaran Bandung Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Takagi","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Institute of Biological Sciences Tokushima University Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Clinical Application, Center of iPS Cell Research and Application Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9826-0203","authenticated-orcid":false,"given":"Takakuni","family":"Maki","sequence":"additional","affiliation":[{"name":"Department of Neurology, Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Graduate School of Medicine, Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awr094"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm747"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0513-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2014.01.013"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.09.002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.12.011"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.05.041"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aws075"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2012.03.049"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408258102"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awm070"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30513-X"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1315710110"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404474101"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3171\/jns.2005.103.1.0038"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12517"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2008.05.006"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.16-0371"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.2217\/whe.11.22"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2011.0489"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2012.12.006"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.07.002"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2015-0261"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1104"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt726"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1826"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(00)00005-8"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199802000-00012"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.019"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2016.10.015"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awt278"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2015.06.006"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2004.09.005"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-016-0347-7"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.24197","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.24197","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:52:51Z","timestamp":1695689571000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.24197"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,7]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1002\/jnr.24197"],"URL":"https:\/\/doi.org\/10.1002\/jnr.24197","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,7]]}}}