{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T05:17:51Z","timestamp":1778044671299,"version":"3.51.4"},"reference-count":100,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T00:00:00Z","timestamp":1614729600000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T00:00:00Z","timestamp":1583193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000912","name":"March of Dimes Foundation","doi-asserted-by":"publisher","award":["5\u2010FY19\u2010111"],"award-info":[{"award-number":["5\u2010FY19\u2010111"]}],"id":[{"id":"10.13039\/100000912","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R00HD084759"],"award-info":[{"award-number":["R00HD084759"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["T32HD087166"],"award-info":[{"award-number":["T32HD087166"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009170","name":"Foreign Agricultural Service","doi-asserted-by":"publisher","award":["Hatch project MICL1018024"],"award-info":[{"award-number":["Hatch project MICL1018024"]}],"id":[{"id":"10.13039\/100009170","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Molecular and behavioral timekeeping is regulated by the circadian system which includes the brain's suprachiasmatic nucleus (SCN) that translates environmental light information into neuronal and endocrine signals aligning peripheral tissue rhythms to the time of day. Despite the critical role of circadian rhythms in fertility, it remains unexplored how circadian rhythms change within reproductive tissues during pregnancy. To determine how estrous cycle and pregnancy impact phase relationships of reproductive tissues, we used PER2::Luciferase (PER2::LUC) circadian reporter mice and determined the time of day of PER2::LUC peak (phase) in the SCN, pituitary, uterus, and ovary. The relationships between reproductive tissue PER2::LUC phases changed throughout the estrous cycle and late pregnancy and were accompanied by changes to PER2::LUC period in the SCN, uterus, and ovary. To determine if the phase relationship adaptations were driven by sex steroids, we asked if progesterone, a hormone involved in estrous cyclicity and pregnancy, could regulate Per2\u2010luciferase expression. Using an\n                    <jats:italic>in vitro<\/jats:italic>\n                    transfection assay, we found that progesterone increased Per2\u2010luciferase expression in immortalized SCN (SCN2.2) and arcuate nucleus (KTAR) cells. In addition, progesterone shortened PER2::LUC period in\n                    <jats:italic>ex vivo<\/jats:italic>\n                    uterine tissue recordings collected during pregnancy. As progesterone dramatically increases during pregnancy, we evaluated wheel\u2010running patterns in PER2::LUC mice. We confirmed that activity levels decrease during pregnancy and found that activity onset was delayed. Although SCN, but not arcuate nucleus, PER2::LUC period changed during late pregnancy, onset of locomotor activity did not correlate with SCN or arcuate nucleus PER2::LUC period.\n                  <\/jats:p>","DOI":"10.1002\/jnr.24606","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T02:16:40Z","timestamp":1583288200000},"page":"294-308","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Circadian rhythms in the mouse reproductive axis during the estrous cycle and pregnancy"],"prefix":"10.1002","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4415-4450","authenticated-orcid":false,"given":"Alexandra M.","family":"Yaw","sequence":"first","affiliation":[{"name":"Department of Animal Science and the Reproductive and Developmental Science Program Michigan State University East Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thu V.","family":"Duong","sequence":"additional","affiliation":[{"name":"Department of Animal Science and the Reproductive and Developmental Science Program Michigan State University East Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duong","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Animal Science and the Reproductive and Developmental Science Program Michigan State University East Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1555-1369","authenticated-orcid":false,"given":"Hanne M.","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Department of Animal Science and the Reproductive and Developmental Science Program Michigan State University East Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0029-7844(95)00119-C"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(81)90073-1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016349109007161"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod20.4.733"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-100-5-1472"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026336505343"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12473"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1071\/BI9710149"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2013.01.003"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-114-3-930"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-102-1-258"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1335.004"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(94)90029-9"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(98)70641-6"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2019.00268"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0190029"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.12729"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei313"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199904)39:1<1::AID-NEU1>3.0.CO;2-F"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(91)90080-8"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3758\/BF03193146"},{"key":"e_1_2_11_23_1","volume-title":"The mouse brain in stereotaxic coordinates","author":"Franklin K. B. J.","year":"2008"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2009-0014"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2017.05.006"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc11-2217"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011-0195"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.06.020"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2012.10.011"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0590249"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-007-9432-7"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02797-2"},{"key":"e_1_2_11_33_1","first-page":"e58352","article-title":"Determination of reproductive competence by confirming pubertal onset and performing a fertility assay in mice and rats","volume":"140","author":"Hoffmann H. M.","year":"2018","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2017.09.004"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2723-15.2016"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-2243(94)90038-8"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI46043"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2016-1294"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(16)32480-2"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.050732"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2005-0243"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddl207"},{"key":"e_1_2_11_43_1","first-page":"60","article-title":"Circadian control of neuroendocrine circuits regulating female reproductive function","volume":"3","author":"Kriegsfeld L. J.","year":"2012","journal-title":"Frontiers in Endocrinology"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1159\/000057339"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.4103\/0300-1652.107549"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1097\/GRF.0b013e31815a5494"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.13085"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.12-225664"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-13-05574.1999"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1209249111"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748730414549767"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1958.0071"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/813764"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2018.03.014"},{"key":"e_1_2_11_55_1","first-page":"291A","article-title":"Chronotype changes during pregnancy","volume":"23","author":"Martin\u2010Fairey C. A.","year":"2016","journal-title":"Reproductive Sciences"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748730419844650"},{"key":"e_1_2_11_57_1","first-page":"359","article-title":"Recent studies on the mechanisms controlling the initiation of lactation","volume":"13","author":"Meites J.","year":"1954","journal-title":"Revue Canadienne de Biologie"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2015-1645"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2013.00195"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.557840"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748730409349059"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/newbio236150a0"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1159\/000122944"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.109322"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11565"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2010.01.007"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719115604729"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2018-00071"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.06.041"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.01.022"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2012.743018"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-19-0302"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-1021"},{"key":"e_1_2_11_74_1","first-page":"154A","article-title":"Time of birth in the rat is gated to the daily light cycle by a circadian mechanism","volume":"17","author":"Reppert S. M.","year":"1983","journal-title":"Pediatric Research"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1982.0030"},{"key":"e_1_2_11_76_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod31.4.656"},{"key":"e_1_2_11_77_1","doi-asserted-by":"publisher","DOI":"10.3109\/07420528709078524"},{"key":"e_1_2_11_78_1","volume-title":"The mouse. Its reproduction and development","author":"Rugh R.","year":"1968"},{"key":"e_1_2_11_79_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.71.090501.142857"},{"key":"e_1_2_11_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/0029-7844(95)00190-3"},{"key":"e_1_2_11_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(91)90364-T"},{"key":"e_1_2_11_82_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2013.00091"},{"key":"e_1_2_11_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2010.06.002"},{"key":"e_1_2_11_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2019.110655"},{"key":"e_1_2_11_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2017.10.012"},{"key":"e_1_2_11_86_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.12104"},{"key":"e_1_2_11_87_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.6.1583"},{"key":"e_1_2_11_88_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2015-1300"},{"key":"e_1_2_11_89_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1980.239.5.R497"},{"key":"e_1_2_11_90_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-95-5-1486"},{"key":"e_1_2_11_91_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.15161"},{"key":"e_1_2_11_92_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0640037"},{"key":"e_1_2_11_93_1","first-page":"852","article-title":"Cellular bioluminescence imaging","volume":"7","author":"Welsh D. K.","year":"2012","journal-title":"Cold Spring Harbor Protocols"},{"key":"e_1_2_11_94_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-15-0405"},{"key":"e_1_2_11_95_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0943"},{"key":"e_1_2_11_96_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2017.03.001"},{"key":"e_1_2_11_97_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308709101"},{"key":"e_1_2_11_98_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-1280"},{"key":"e_1_2_11_99_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2006.09.005"},{"key":"e_1_2_11_100_1","first-page":"799","article-title":"Regulation of reproduction by the circadian rhythms","volume":"68","author":"Zhang W. X.","year":"2016","journal-title":"Acta Physiologica Sinica"},{"key":"e_1_2_11_101_1","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.2417"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.24606","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.24606","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jnr.24606","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/jnr.24606","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.24606","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T09:29:10Z","timestamp":1693819750000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.24606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,3]]},"references-count":100,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1002\/jnr.24606"],"URL":"https:\/\/doi.org\/10.1002\/jnr.24606","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/JNR.24606\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24606\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24606\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24606\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24606\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24606\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24606\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,3]]},"assertion":[{"value":"2019-10-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}