{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:29:17Z","timestamp":1781224157252,"version":"3.54.1"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T00:00:00Z","timestamp":1623283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Several eye diseases, for example, retinal artery occlusion, diabetic retinopathy, and glaucoma, are associated with retinal hypoxia. The lack of oxygen in the retina, especially in retinal ganglion cells (RGCs), causes cell damage up to cell degeneration and leads to blindness. Using multielectrode array recordings, an\n                    <jats:italic>ex vivo<\/jats:italic>\n                    hypoxia acute model was established to analyze the electrical activity of murine wild\u2010type retinae under hypoxic stress conditions. Hypoxia was induced by exchanging the perfusion with oxygen\u2010saturated medium by nitrogen\u2010saturated medium. Hypoxic periods of 0\u00a0min (control) up to 60\u00a0min were tested on the retinae of adult female C57BL\/6J mice. The electrical RGC activity vanished during hypoxia, but conditionally returned after the reestablishment of conventional test conditions. With increasing duration of hypoxia, the returning RGC activity decreased. After a hypoxic period of 30\u00a0min and a subsequent recovery time of 30\u00a0min, 59.43\u00a0\u00b1\u00a011.35% of the initially active channels showed a restored RGC activity. The survival rate of retinal cells after hypoxic stress was analyzed by a live\/dead staining assay using two\u2010photon laser scanning microscopy. For detailed information about molecular changes caused by hypoxia, a microarray gene expression analysis was performed. Furthermore, the effect of 2\u2010aminoethanesulfonic acid (taurine, 1\u00a0mM) on retinae under hypoxic stress was tested. Treatment with taurine resulted in an increase in the RGC response rate after hypoxia and also increased the survival rate of retinal cells under hypoxic stress, confirming its potential as promising candidate for neuroprotective therapies of eye diseases.\n                  <\/jats:p>","DOI":"10.1002\/jnr.24899","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T22:13:05Z","timestamp":1623363185000},"page":"2172-2187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A multielectrode array\u2010based hypoxia model for the analysis of electrical activity in murine retinae"],"prefix":"10.1002","volume":"99","author":[{"given":"Claudia","family":"Ingensiep","sequence":"first","affiliation":[{"name":"Department of Ophthalmology University Hospital RWTH Aachen Aachen 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