{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T02:31:46Z","timestamp":1782441106496,"version":"3.54.5"},"reference-count":57,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T00:00:00Z","timestamp":1626739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100003146","name":"Morton Cure Paralysis Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100003146","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008191","name":"Wings for Life","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008191","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The cytoskeleton of ependymal cells is fundamental to organize and maintain the normal architecture of the central canal (CC). However, little is known about the plasticity of cytoskeletal components after spinal cord injury. Here, we focus on the structural organization of the cytoskeleton of ependymal cells in the normal and injured spinal cord of mice (both females and males) using immunohistochemical and electron microscopy techniques. We found that in uninjured animals, the actin cytoskeleton (as revealed by phalloidin staining) was arranged following the typical pattern of polarized epithelial cells with conspicuous actin pools located in the apical domain of ependymal cells. Transmission electron microscopy images showed microvilli tufts, long cilia, and characteristic intercellular membrane specializations. After spinal cord injury, F\u2010actin rearrangements paralleled by fine structural modifications of the apical domain of ependymal cells were observed. These changes involved disruptions of the apical actin pools as well as fine structural modifications of the microvilli tufts. When comparing the control and injured spinal cords, we also found modifications in the expression of vimentin and glial fibrillary acidic protein (GFAP). After injury, vimentin expression disappeared from the most apical domains of ependymal cells but the number of GFAP\u2010expressing cells within the CC increased. As in other polarized epithelia, the plastic changes in the cytoskeleton may be critically involved in the reaction of ependymal cells following a traumatic injury of the spinal cord.<\/jats:p>","DOI":"10.1002\/jnr.24918","type":"journal-article","created":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T08:21:47Z","timestamp":1626769307000},"page":"2592-2609","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The ependymal cell cytoskeleton in the normal and injured spinal cord of mice"],"prefix":"10.1002","volume":"99","author":[{"given":"Omar","family":"Trujillo\u2010Cen\u00f3z","sequence":"first","affiliation":[{"name":"Departamento de Neurofisiolog\u00eda Celular y Molecular Instituto de Investigaciones Biol\u00f3gicas Clemente Estable Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda I.","family":"Rehermann","sequence":"additional","affiliation":[{"name":"Departamento de Neurofisiolog\u00eda Celular y Molecular Instituto de Investigaciones Biol\u00f3gicas Clemente Estable Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Maciel","sequence":"additional","affiliation":[{"name":"Departamento de Neurofisiolog\u00eda Celular y Molecular Instituto de Investigaciones Biol\u00f3gicas Clemente Estable Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda V.","family":"Falco","sequence":"additional","affiliation":[{"name":"Departamento de Neurofisiolog\u00eda Celular y Molecular Instituto de Investigaciones Biol\u00f3gicas Clemente Estable Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Fabbiani","sequence":"additional","affiliation":[{"name":"Departamento de Neurofisiolog\u00eda Celular y Molecular Instituto de Investigaciones Biol\u00f3gicas Clemente Estable Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal E.","family":"Russo","sequence":"additional","affiliation":[{"name":"Departamento de Neurofisiolog\u00eda Celular y Molecular Instituto de Investigaciones Biol\u00f3gicas Clemente Estable Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23104"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00018.2013"},{"key":"e_1_2_11_4_1","first-page":"55","article-title":"Ependyma of the central canal of the rat spinal cord: A light and transmission electron microscopic study","volume":"152","author":"Bruni J. E.","year":"1987","journal-title":"Journal of Anatomy"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.188599"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22642"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2015.09.009"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1998.0680"},{"key":"e_1_2_11_9_1","first-page":"421","volume-title":"The cell. A molecular approach","author":"Cooper G.","year":"2000"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201407015"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(75)90196-8"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2016.04.001"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12029-4"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI38339"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100617-062534"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0736-5748(93)90020-E"},{"key":"e_1_2_11_17_1","first-page":"428","article-title":"Organization of actin, myosin and intermediate filaments in the brush border of intestinal epithelial cells","volume":"94","author":"Hirokawa N.","year":"1982","journal-title":"Journal of Cell Biology"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a021642"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2015.02.004"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2018.0227"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001780205"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.051508.135600"},{"key":"e_1_2_11_23_1","series-title":"The Frontiers Collection","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1007\/3-540-33654-0","volume-title":"The thermodynamic machinery of life","author":"Kurzynski M.","year":"2006"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4689-05.2006"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2018.04.017"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.08.045"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2009.953646"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb8795"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R115.640359"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0224-08.2008"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-62817-2_13"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02439"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4160-5766-6.00024-8"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0060182"},{"key":"e_1_2_11_35_1","first-page":"465","article-title":"Effects of cytochalasin B on the ependyma","volume":"3","author":"Mestres P.","year":"1980","journal-title":"Scanning Electron Microscopy"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2011.01.005"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2019.11.008"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.10.011"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12354"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1136(20010101)33:1<72::AID-GLIA1007>3.0.CO;2-A"},{"key":"e_1_2_11_41_1","first-page":"312","volume-title":"The fine structure of the nervous system: Neurons and their supporting cells","author":"Peters A.","year":"1991"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1665"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41122"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1242576"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.226"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2017.00081"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2020.06.004"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14930"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.07.055"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a018267"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-101011-155801"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.99.1.76s"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03168"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218446110"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2015.00016"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.278.6.F886"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200804062"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.24918","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jnr.24918","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.24918","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T10:29:07Z","timestamp":1692959347000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.24918"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,20]]},"references-count":57,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["10.1002\/jnr.24918"],"URL":"https:\/\/doi.org\/10.1002\/jnr.24918","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/JNR.24918\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24918\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24918\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24918\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24918\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24918\/v2\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24918\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24918\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.24918\/v1\/review1","asserted-by":"object"}]},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,20]]},"assertion":[{"value":"2020-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}