{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:10:40Z","timestamp":1791403840389,"version":"4.3.3"},"reference-count":64,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:00:00Z","timestamp":1687996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY20H150001"],"award-info":[{"award-number":["LY20H150001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mild traumatic brain injury (mTBI) is a clinically highly heterogeneous neurological disorder, none of the existing animal models can replicate the entire sequelae. This study aimed to develop a modified closed head injury (CHI) model of repeated mTBI (rmTBI) for investigating Ca\n                    <jats:sup>2+<\/jats:sup>\n                    fluctuations of the affected neural network, the alternations of electrophysiology, and behavioral dysfunctions. The transcranial Ca\n                    <jats:sup>2+<\/jats:sup>\n                    study protocol includes AAV\u2010GCaMP6s infection in the right motor cortex, thinned\u2010skull preparation, and two\u2010photon laser scanning microscopy (TPLSM) imaging. The CHI rmTBI model is fabricated using the thinned\u2010skull site and applying 2.0\u2009atm fluid percussion with 48\u2010h interval. The neurological dysfunction, minor motor performance, evident mood, spatial working, and reference deficits we found in this study mimic the clinically relevant syndromes after mTBI. Besides, our study revealed that there was a trend of transition from Ca\n                    <jats:sup>2+<\/jats:sup>\n                    singlepeak to multipeak and plateau, and the total Ca\n                    <jats:sup>2+<\/jats:sup>\n                    activities of multipeaks and plateaus (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.001 vs. pre\u2010rmTBI value) were significantly increased in ipsilateral layer 2\/3 motor neurons after rm TBI. In parallel, there is a low\u2010frequency power shift from delta to theta band (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.01 vs. control) in the ipsilateral layer 2\/3 of motor cortex of the rmTBI mice, and the overall firing rates significantly increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.01 vs. control). Moreover, rmTBI causes slight cortical and hippocampal neuron damage and possibly induces neurogenesis in the dentate gyrus (DG). The alternations of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    and electrophysiological characteristics in layer 2\/3 neuronal network, histopathological changes, and possible neurogenesis may play concertedly and partially contribute to the functional outcome post\u2010rmTBI.\n                  <\/jats:p>","DOI":"10.1002\/jnr.25227","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T05:35:56Z","timestamp":1688016956000},"page":"1633-1650","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Modified mouse model of repeated mild traumatic brain injury through a thinned\u2010skull window and fluid percussion"],"prefix":"10.1002","volume":"101","author":[{"given":"Yuncheng","family":"Liu","sequence":"first","affiliation":[{"name":"College of Physical Education and Health Sciences Zhejiang Normal University  Jinhua 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