{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T23:48:25Z","timestamp":1782949705660,"version":"3.54.5"},"reference-count":96,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007543","name":"Grantov\u00e1 Agentura, Univerzita Karlova","doi-asserted-by":"publisher","award":["1210217"],"award-info":[{"award-number":["1210217"]}],"id":[{"id":"10.13039\/100007543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008579","name":"P\u0159\u00edrodov\u011bdeck\u00e1 Fakulta, Univerzita Karlova","doi-asserted-by":"publisher","award":["SVV\u2010260571\/2020"],"award-info":[{"award-number":["SVV\u2010260571\/2020"]}],"id":[{"id":"10.13039\/100008579","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The circadian clock is one of the most important homeostatic systems regulating the majority of physiological functions. Its proper development contributes significantly to the maintenance of health in adulthood. Methadone is recommended for the treatment of opioid use disorders during pregnancy, increasing the number of children prenatally exposed to long\u2010acting opioids. Although early\u2010life opioid exposure has been studied for a number of behavioral and physiological changes observed later in life, information on the relationship between the effects of methadone exposure and circadian system development is lacking. Using a rat model, we investigated the effects of prenatal and early postnatal methadone administration on the maturation of the circadian clockwork in the suprachiasmatic nucleus (SCN) and liver, the rhythm of aralkylamine N\u2010acetyltransferase (AA\u2010NAT) activity in the pineal gland, and gene expression in the livers of 20\u2010day\u2010old rats. Our data show that repeated administration of methadone to pregnant and lactating mothers has significant effect on rhythmic gene expression in the SCN and livers and on the rhythm of AA\u2010NAT in the offspring. Similar to previous studies with morphine, the rhythm amplitudes of the clock genes in the SCN and liver were unchanged or enhanced. However, six of seven specific genes in the liver showed significant downregulation of their expression, compared to the controls in at least one experimental group. Importantly, the amplitude of the AA\u2010NAT rhythm was significantly reduced in all methadone\u2010treated groups. As there is a strong correlation with melatonin levels, this result could be of importance for clinical practice.<\/jats:p>","DOI":"10.1002\/jnr.25236","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T03:25:53Z","timestamp":1691465153000},"page":"1737-1756","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Methadone administered to rat dams during pregnancy and lactation affects the circadian rhythms of their pups"],"prefix":"10.1002","volume":"101","author":[{"given":"Dominika","family":"Pa\u010desov\u00e1","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Spi\u0161sk\u00e1","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u0159\u00ed","family":"Novotn\u00fd","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2693-2143","authenticated-orcid":false,"given":"Zde\u0148ka","family":"Bendov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Science Charles University  Prague Czech Republic"},{"name":"National Institute of Mental Health  Klecany Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1479\u2010828X.2009.01010.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424\u2010011\u20100986\u20106"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092\u20108674(00)80495\u2010X"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.03.051"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201004\u20100496"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1419"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1940\u20100640\u20109\u201019"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.11.6979"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24906"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/jp.2015.22"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756\u2010200005150\u201000019"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2001.280.4.R1023"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2006\u201017.2017"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.2147\/NDT.S70585"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00498254.2020.1737890"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.292.5522.1728"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/3204504"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuchi.2015.03.002"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742\u20104682\u201011\u201016"},{"issue":"1","key":"e_1_2_10_21_1","first-page":"33","article-title":"The effect of opioids on sleep architecture","volume":"3","author":"Dimsdale J. E.","year":"2007","journal-title":"Journal of Clinical Sleep Medicine"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601126"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748730408322635"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.25600"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/10550880903436010"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304664200"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246\u2010200101000\u201000021"},{"issue":"12","key":"e_1_2_10_28_1","first-page":"1689","article-title":"Methadone exposure during lactation","volume":"54","author":"Glatstein M. M.","year":"2008","journal-title":"Canadian Family Physician Medecin de Famille Canadien"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2020.113633"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010079x.1998.tb00533.x"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010079x.1992.tb00066.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.06.084"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165\u20103806(90)90056\u20105"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432\u2010020\u201003485\u20103"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1037\/e677012010-001"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(00)03409\u20107"},{"issue":"4","key":"e_1_2_10_37_1","first-page":"429","article-title":"Methadone and heroin during pregnancy: A review of behavioral effects in human and animal offspring","volume":"4","author":"Hutchings D. E.","year":"1982","journal-title":"Neurobehavioral Toxicology and Teratology"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2012.12.035"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1177\/1078155209337665"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0095-5108(18)30077-0"},{"key":"e_1_2_10_41_1","first-page":"307","article-title":"The melatonin rhythm\u2010generating enzyme: Molecular regulation of serotonin N\u2010acetyltransferase in the pineal gland","volume":"52","author":"Klein D. C.","year":"1997","journal-title":"Recent Progress in Hormone Research"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6928a1"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2020.108008"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines8120579"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2018.00382"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2010.03.020"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.2147\/JPR.S125264"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10146"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"issue":"1","key":"e_1_2_10_50_1","first-page":"209","article-title":"Morphine phase\u2010shifts circadian rhythms in mice: Role of behavioural activation","volume":"7","author":"Marchant E. G.","year":"1995","journal-title":"Neuroreport"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.15211"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.14279"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1159\/000112395"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0006\u20108993(00)02317\u20109"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.09-06-02151.1989"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23673"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/life11101105"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/216494a0"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.15412\/J.BCN.03070401"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873\u20103468.13328"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010bioeng\u2010082120\u2010034725"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976\u20100105.177703"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2003.11.023"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031\u2010009\u20109312\u20106"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.12400"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2004.09.008"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1177\/17448069211003375"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12462"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.933183"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2021.09.016"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13152"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1097\/JCP.0b013e31823f91d1"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568026621666210521165532"},{"issue":"1","key":"e_1_2_10_74_1","first-page":"57","article-title":"Divergent regulation of cytochrome P450 enzymes by morphine and pethidine: A neuroendocrine mechanism?","volume":"47","author":"Rane A.","year":"1995","journal-title":"Molecular Pharmacology"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0892\u20100362(97)00018\u20104"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2019.10.018"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1080\/07420520500521962"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600\u2010079x.2001.310408.x"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401149101"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1511351113"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165\u20103806(93)90205\u2010o"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43188\u2010019\u201000019\u2010z"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M602374200"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.10.7708"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010079x.1997.tb00346.x"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460\u20109568.2005.04131.x"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1159\/000365074"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-09-05393.1994"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2007.03.070"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.19.9201"},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ntt.2010.01.007"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03393"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2014.09.008"},{"key":"e_1_2_10_94_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020\u2010021\u201000325\u2010z"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102054"},{"key":"e_1_2_10_96_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2019.01.010"},{"key":"e_1_2_10_97_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.jom.0000145168.21614.21"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.25236","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T09:55:56Z","timestamp":1695808556000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.25236"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,8]]},"references-count":96,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1002\/jnr.25236"],"URL":"https:\/\/doi.org\/10.1002\/jnr.25236","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/JNR.25236\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25236\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,8]]},"assertion":[{"value":"2023-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}