{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:19:25Z","timestamp":1791555565692,"version":"4.3.4"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T00:00:00Z","timestamp":1710979200000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["733050816"],"award-info":[{"award-number":["733050816"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alzheimer's disease (AD) is a progressive neurodegenerative disease and the most common cause of dementia, characterized by deposition of extracellular amyloid\u2010beta (A\u03b2) aggregates and intraneuronal hyperphosphorylated Tau. Many AD risk genes, identified in genome\u2010wide association studies (GWAS), are expressed in microglia, the innate immune cells of the central nervous system. Specific subtypes of microglia emerged in relation to AD pathology, such as disease\u2010associated microglia (DAMs), which increased in number with age in amyloid mouse models and in human AD cases. However, the initial transcriptional changes in these microglia in response to amyloid are still unknown. Here, to determine early changes in microglia gene expression, hippocampal microglia from male APPswe\/PS1dE9 (APP\/PS1) mice and wild\u2010type littermates were isolated and analyzed by RNA sequencing (RNA\u2010seq). By bulk RNA\u2010seq, transcriptomic changes were detected in hippocampal microglia from 6\u2010months\u2010old APP\/PS1 mice. By performing single\u2010cell RNA\u2010seq of CD11c\u2010positive and negative microglia from 6\u2010months\u2010old APP\/PS1 mice and analysis of the transcriptional trajectory from homeostatic to CD11c\u2010positive microglia, we identified a set of genes that potentially reflect the initial response of microglia to A\u03b2.<\/jats:p>","DOI":"10.1002\/jnr.25295","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T02:54:21Z","timestamp":1711076061000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Early amyloid\u2010induced changes in microglia gene expression in male\n                    <scp>APP<\/scp>\n                    \/\n                    <scp>PS1<\/scp>\n                    mice"],"prefix":"10.1002","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1430-4211","authenticated-orcid":false,"given":"Takuya","family":"Oshima","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Section Molecular Neurobiology University Medical Center Groningen, University of Groningen  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4832-2597","authenticated-orcid":false,"given":"Mandy S. 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