{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:10:20Z","timestamp":1772118620284,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T00:00:00Z","timestamp":1712707200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014197","name":"Stiftelsen Mary von Sydows, f\u00f6dd Wijk, donationsfond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014197","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003748","name":"Svenska S\u00e4llskapet f\u00f6r Medicinsk Forskning","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003748","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003745","name":"Stiftelserna Wilhelm och Martina Lundgrens","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003745","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2021\u201001872"],"award-info":[{"award-number":["2021\u201001872"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003792","name":"Hj\u00e4rnfonden","doi-asserted-by":"publisher","award":["FO2022\u20100110"],"award-info":[{"award-number":["FO2022\u20100110"]}],"id":[{"id":"10.13039\/501100003792","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>There is a need for new treatments to reduce brain injuries derived from neonatal hypoxia\/ischemia. The only viable option used in the clinic today in infants born at term is therapeutic hypothermia, which has a limited efficacy. Treatments with exogenous RNase have shown great promise in a range of different adult animal models including stroke, ischemia\/reperfusion injury, or experimental heart transplantation, often by conferring vascular protective and anti\u2010inflammatory effects. However, any neuroprotective function of RNase treatment in the neonate remains unknown. Using a well\u2010established model of neonatal hypoxic\/ischemic brain injury, we evaluated the influence of RNase treatment on RNase activity, gray and white matter tissue loss, blood\u2013brain barrier function, as well as levels and expression of inflammatory cytokines in the brain up to 6\u2009h after the injury using multiplex immunoassay and RT\u2010PCR. Intraperitoneal treatment with RNase increased RNase activity in both plasma and cerebropinal fluids. The RNase treatment resulted in a reduction of brain tissue loss but did not affect the blood\u2013brain barrier function and had only a minor modulatory effect on the inflammatory response. It is concluded that RNase treatment may be promising as a neuroprotective regimen, whereas the mechanistic effects of this treatment appear to be different in the neonate compared to the adult and need further investigation.<\/jats:p>","DOI":"10.1002\/jnr.25329","type":"journal-article","created":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T05:27:03Z","timestamp":1712726823000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Treatment with\n                    <scp>RNase<\/scp>\n                    alleviates brain injury but not neuroinflammation in neonatal hypoxia\/ischemia"],"prefix":"10.1002","volume":"102","author":[{"given":"Rozita H","family":"Anderberg","sequence":"first","affiliation":[{"name":"Institute of Neuroscience and Physiology, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. Axel","family":"Andersson","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience and Physiology, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2177-0587","authenticated-orcid":false,"given":"Valentina","family":"Bucher","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Institute of Clinical Sciences, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus T.","family":"Preissner","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Medical School, Kerckhoff\u2010Heart Research Institute Justus\u2010Liebig\u2010University  Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carina","family":"Mallard","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience and Physiology, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Joakim","family":"Ek","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience and Physiology, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201900451R"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.113.000683"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2015.00198"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0197-2456(90)90005-M"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2014.255"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra041996"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-014-1850-8"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-08-040691"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH10-06-0345"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000449032"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199802000-00014"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02626"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000329710"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01317-y"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.116.003429"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X17746132"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-09326-2"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.576736"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1478-4505-5-4"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(01)00030-8"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5553486"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2018.00428"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-018-1321-4"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2015.04.060"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.02.094"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.18088"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1397344"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0190002"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2018.4182"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjos-2020-100115"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH-16-11-0858"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(00)00111-5"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)41154-2"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awy220"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2020.108088"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.116.004541"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2019.00254"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.2174\/156720209787466037"},{"key":"e_1_2_10_43_1","first-page":"5","article-title":"The bicinchoninic acid (BCA) assay for protein quantitation","volume":"32","author":"Walker J. M.","year":"1994","journal-title":"Methods in Molecular Biology"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21066"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2016.09.007"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.25329","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,21]],"date-time":"2024-09-21T04:10:06Z","timestamp":1726891806000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.25329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":44,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/jnr.25329"],"URL":"https:\/\/doi.org\/10.1002\/jnr.25329","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/JNR.25329\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25329\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25329\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25329\/v4\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25329\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25329\/v4\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25329\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25329\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25329\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25329\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JNR.25329\/v2\/review1","asserted-by":"object"}]},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"2023-05-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e25329"}}