{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T05:25:53Z","timestamp":1784784353244,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T00:00:00Z","timestamp":1728000000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K08859"],"award-info":[{"award-number":["18K08859"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K08925"],"award-info":[{"award-number":["21K08925"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H06276"],"award-info":[{"award-number":["16H06276"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019890","name":"University of Miyazaki Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019890","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2024,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Voltage\u2010gated sodium channels, including Na\n V<\/jats:sub>\n 1.7, Na\n V<\/jats:sub>\n 1.8, and Na\n V<\/jats:sub>\n 1.9, play important roles in pain transmission and chronic pain development. However, the specific mechanisms of their action remain unclear, highlighting the need for in\u00a0vivo stimulation studies of these channels. Optogenetics, a novel technique for targeting the activation or inhibition of specific neural circuits using light, offers a promising solution. In our previous study, we used optogenetics to selectively excite Na\n V<\/jats:sub>\n 1.7\u2010expressing neurons in the dorsal root ganglion of mice to induce nocifensive behavior. Here, we further characterize the impact of nocifensive behavior by activation of Na\n V<\/jats:sub>\n 1.7, Na\n V<\/jats:sub>\n 1.8, or Na\n V<\/jats:sub>\n 1.9\u2010expressing neurons. Using CRISPR\/Cas9\u2010mediated homologous recombination, Na\n V<\/jats:sub>\n 1.7\u2013iCre, Na\n V<\/jats:sub>\n 1.8\u2013iCre, or Na\n V<\/jats:sub>\n 1.9\u2013iCre mice expressing iCre recombinase under the control of the endogenous Na\n V<\/jats:sub>\n 1.7, Na\n V<\/jats:sub>\n 1.8, or Na\n V<\/jats:sub>\n 1.9 gene promoter were produced. These mice were then bred with channelrhodopsin\u20102 (ChR2) Cre\u2013reporter Ai32 mice to obtain Na\n V<\/jats:sub>\n 1.7\u2013ChR2, Na\n V<\/jats:sub>\n 1.8\u2013ChR2, or Na\n V<\/jats:sub>\n 1.9\u2013ChR2 mice. Blue light exposure triggered paw withdrawal in all mice, with the strongest response in Na\n V<\/jats:sub>\n 1.8\u2013ChR2 mice. These light sensitivity differences observed across Na\n V<\/jats:sub>\n 1.x\u2013ChR2 mice may be dependent on ChR2 expression or reflect the inherent disparities in their pain transmission roles. In conclusion, we have generated noninvasive pain models, with optically activated peripheral nociceptors. We believe that studies using optogenetics will further elucidate the role of sodium channel subtypes in pain transmission.\n <\/jats:p>","DOI":"10.1002\/jnr.25386","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T05:27:42Z","timestamp":1728019662000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of Nocifensive Behavior in\n \n Na\n V<\/sub>\n 1\n <\/scp>\n .7\u2013,\n \n Na\n V<\/sub>\n 1\n <\/scp>\n .8\u2013, and\n \n Na\n V<\/sub>\n 1\n <\/scp>\n .9\u2013Channelrhodopsin\u20102 Mice by Selective Optogenetic Activation of Targeted Sodium Channel Subtype\u2010Expressing Afferents"],"prefix":"10.1002","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2660-2179","authenticated-orcid":false,"given":"Toyoaki","family":"Maruta","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Faculty of Medicine University of Miyazaki Miyazaki 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