{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T17:03:13Z","timestamp":1706202193083},"reference-count":33,"publisher":"Wiley","issue":"2-3","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":8319,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1982,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Survival in monolayer culture of 4\u2010day (stage 23) chick embryo lumbar spinal cord neurons can be regulated by two opposing activities. One, spinal neuronotrophic activity, promotes neuronal survival; and the other, spinal neuronotoxic activity, eliminates the neurons from the culture even when the trophic support is present at an optimal concentration. Quantitative microbioassays for each activity are presented and used to measure the relative amounts of each agent within different sources including glial, muscle, and spinal cord cell\u2010conditioned media and fluid collected from peripheral and central nervous tissue lesions. Although both activities were present in all of the sources tested, their concentrations in the wound fluids were orders of magnitude greater than in the conditioned media. The fluid\u2010derived trophic activities were inactivated by heat and trypsin and nondialyzable, whereas all of the conditioned media\u2010derived trophic activities were heat\u2010 and trypsin\u2010resistant and dialyzable.<\/jats:p>","DOI":"10.1002\/jnr.490080214","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T10:42:15Z","timestamp":1104576135000},"page":"241-250","source":"Crossref","is-referenced-by-count":31,"title":["Comparative features of spinal neuronotrophic factors in fluids collected in vitro and in vivo"],"prefix":"10.1002","volume":"8","author":[{"given":"Marston","family":"Manthorpe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank M.","family":"Longo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvio","family":"Varon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(81)90105-0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(79)90302-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","unstructured":"AdlerR ManthorpeM VaronS(1982): Lectin reactivity of PNPF a polyornithine\u2010binding neurite promoting factor.Dev Brain Res(in press).","DOI":"10.1016\/0165-3806(82)90175-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7403847"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(80)90295-4"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.77.1.83"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/286025a0"},{"key":"e_1_2_1_9_1","first-page":"552","article-title":"Nerve growth activities in rat peripheral nerve","volume":"7","author":"Ebendal T","year":"1981","journal-title":"Soc Neurosci Abstr"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.4.2625"},{"key":"e_1_2_1_11_1","first-page":"511","volume-title":"Methods in Enzymol","author":"Konigsburg IR","year":"1979"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1968.48.3.534"},{"key":"e_1_2_1_13_1","unstructured":"LongoFM ManthorpeM SkaperSD LundborgG VaronS(1982a): Neuronotrophic activities in fluid accumulated in vivo within silicone nerve regeneration chambers.Brain Res(in press)."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(82)90027-X"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90618-7"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01208512"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(80)90410-2"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1982.tb12552.x"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(81)90408-X"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","unstructured":"ManthorpeM LuytenW LongoFM VaronS(1982a):Endogenous and exogenous factors support neuronal survival and choline acetyltransferase activity in embryonic spinal cord cultures. 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