{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:50:50Z","timestamp":1791175850074,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":6858,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cytopathic effects caused by Theiler viruses to myelinated organotypic spinal cord cultures was studied by light and electron microscopy. Heavily myelinated cultures, 2\u20133 weeks in vitro were infected with WW and GD VII viruses. Mock infection served as control. On light microscopy cytopathic effects and demyelination become evident about 16\u201317 hr after infection. Demyelination observed in WW virus\u2010infected cultures was much more pronounced than in cultures infected with GD VII viruses. The myelin in mock\u2010infected cultures remained undamaged.<\/jats:p><jats:p>Electron microscopy revealed that in control cultures cells were intact, exhibiting numerous synapses and a network of axons enwrapped by multilayered myelin sheaths. Virus\u2010infected spinal cord slices showed that a more severe cytotoxicity was caused by GD VII virus than by WW virus. The cytopathology included accumulation of cytoplasmatic vacuoles, margination of chromatin, synapse and cell disintegration, and various degrees of demyelination. Several GD VII virions were observed, arranged in crystalline arrays, mainly in electron\u2010opaqe cells, but not within axons. WW virions on the other hand were only occasionally encountered.<\/jats:p>","DOI":"10.1002\/jnr.490160408","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T01:28:38Z","timestamp":1104542918000},"page":"671-681","source":"Crossref","is-referenced-by-count":12,"title":["In vitro cytotoxicity and demyelination induced by theiler viruses in cultures of spinal cord slices"],"prefix":"10.1002","volume":"16","author":[{"given":"A.","family":"Shahar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Frankel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"David","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Friedmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90040-4"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1128\/iai.35.3.1133-1138.1982","article-title":"Uncoupled relationship between demyelination and primary infection of myelinated cells in Theiler's virus encephalomyelitis","volume":"35","author":"Dal Canto MC","year":"1982","journal-title":"Infect Immun"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(85)90003-7"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(79)90172-2"},{"key":"e_1_2_1_6_1","first-page":"101","article-title":"Schwann cell remyelination and recurrent demyelination in central nervous system of mice infected with attenuated Theiler's virus","volume":"98","author":"Dal Canto MC","year":"1980","journal-title":"Am J pathol"},{"key":"e_1_2_1_7_1","first-page":"626","article-title":"Primary demyelination in Theiler's virus infection: An ultrastructural study","volume":"33","author":"Dal Canto MC","year":"1985","journal-title":"Lab Invest"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410110202"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.96.6.517"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490150202"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1768-322X.1984.tb00259.x"},{"key":"e_1_2_1_12_1","first-page":"43","article-title":"Theiler's virus infection. A model for multiple sclerosis","volume":"31","author":"Friedmann A","year":"1985","journal-title":"Prog Med Virol"},{"key":"e_1_2_1_13_1","first-page":"298","article-title":"Pathogenesis of Theiler's viruses (TV): Sensitivity of central nervous system cells (CNS) to TV infection, and effect of anti I\u2010A monoclonal antibodies on TV infection","volume":"10","author":"Friedmann A","year":"1986","journal-title":"J Cell Biochem"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(81)90003-0"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-5250-4_37"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1128\/iai.11.5.1147-1155.1975","article-title":"Theiler's virus infection of mice. An unusual biphasic disease process leading to demyelination","volume":"11","author":"Lipton HL","year":"1975","journal-title":"Infect Immun"},{"key":"e_1_2_1_17_1","first-page":"679","volume-title":"ICN\u2010UCLA Symposia on Molecular and Cellular Biology","author":"Lipton HL","year":"1978"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-46-1-169"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.176726"},{"key":"e_1_2_1_20_1","first-page":"505","volume-title":"Schlessinger: Microbiology","author":"Lipton HL","year":"1977"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.4049\/jimmunol.98.1.46","article-title":"The pathogenesis of Theiler's GDVII encephalitis virus infection in mice as studied by immunofluorescent and infectivity titration","volume":"98","author":"Liu C","year":"1967","journal-title":"J Immun"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1128\/jvi.45.2.496-504.1983","article-title":"Protein induced in tissue culture by four isolated of Theiler's murine encephalomyelitis virus","volume":"45","author":"Lorch Y","year":"1983","journal-title":"J Virol"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.40.2.560-567.1981"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(81)90140-4"},{"key":"e_1_2_1_25_1","unstructured":"RodriguezM LafuseWP LeikowitzJ PanolCS(in press):Partial suppression of Theiler's virus\u2010induced demyelination in vivo by administration of monoclonal antibodies to immune response gene products (Ia antigens). Neurology."},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(82)90057-1"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-92-22487"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.65.5.705"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.72.1.49"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00698267"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.490160408","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.490160408","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T19:31:03Z","timestamp":1697830263000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.490160408"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1986,1]]}},"alternative-id":["10.1002\/jnr.490160408"],"URL":"https:\/\/doi.org\/10.1002\/jnr.490160408","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}