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The RC3 protein amino terminus contains a cysteine\u2010rich domain similar to those found in snake venom neurotoxins; the carboxyl terminus contains a collagen\u2010like motif that may function in the assembly of RC3 subunits into a multimeric protein. Western blot experiments with an antiserum to a synthetic peptide corresponding to 27 residues of the 78 residue sequence identify an immunoreactive polypeptide with 18 kDa gel mobility that is likely to correspond to the RC3 protein. Northern blot analysis and in situ hybridization experiments show that RC3 mRNA is highly enriched in rat brain, with restricted expression in neuronal subsets primarily in the cortex, striatum, and hippocampus as well as certain nuclei within the thalamus, hypothalamus, the olfactory bulb.<\/jats:p>","DOI":"10.1002\/jnr.490260402","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T05:38:31Z","timestamp":1104557911000},"page":"397-408","source":"Crossref","is-referenced-by-count":167,"title":["Subtractive cDNA cloning of RC3, a rodent cortex\u2010enriched mRNA encoding a novel 78 residue protein"],"prefix":"10.1002","volume":"26","author":[{"given":"J. B.","family":"Watson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. F.","family":"Battenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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