{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:05:14Z","timestamp":1760954714140},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":5001,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1991,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous work by others has demonstrated that neocortical injury results in the induction of c\u2010<jats:italic>fos<\/jats:italic> proto\u2010oncogene both at and distal to the site of injury. However, secondary effects of focal brain injury, such as spreading depression and seizure activity, also have been shown to induce the expression of c\u2010<jats:italic>fos<\/jats:italic>. Thus, it is unclear whether the stimulus inducing c\u2010<jats:italic>fos<\/jats:italic> expression after generalized brain trauma is direct neuronal injury or associated, secondary effects of injury. In this study, we tested the hypothesis that a specific axonal disconnection would induce the expression of c\u2010<jats:italic>fos<\/jats:italic> proto\u2010oncogene in the injured neurons. The injury paradigm that was used was peripheral axotomy of rodent facial motoneurons. The right facial nerve was severed distal to the stylomastoid foramen, with the left side serving as an internal control. As positive controls, in a <jats:italic>separate<\/jats:italic> group of animals, seizures were invoked using bicucullin administered intraperitoneally. At the end of postoperative survival times ranging from 30 min to 24 hr, the animals were sacrificed. For northern blot analysis using a c\u2010<jats:italic>fos<\/jats:italic> cDNA probe, total RNA was isolated from the dissected facial nuclear groups in the injury experiments, or whole brain and neocortex in the seizure experiments. For immunocytochemical analysis using a battery of c\u2010<jats:italic>fos<\/jats:italic> antibodies, the animals were perfused with paraformaldehyde and processed for routine light microscope immunocytochemistry. In the bicucullin\u2010injected animals, c\u2010<jats:italic>fos<\/jats:italic> mRNA was massively induced in whole brain in a manner proportional to the overall level of gross seizure activity. The localization of the c\u2010<jats:italic>fos<\/jats:italic> expression occurred primarily in neurons within the neocortex. In the axotomized facial motoneurons, no induction of c\u2010<jats:italic>fos<\/jats:italic> mRNA or protein was found to occur at any of the timepoints examined and no basal expression of c\u2010<jats:italic>fos<\/jats:italic> was observed in the uninjured motoneurons. The results suggest that the rapid induction of c\u2010<jats:italic>fos<\/jats:italic> proto\u2010oncogene product is not a specific component of the nerve cell body response to injury.<\/jats:p>","DOI":"10.1002\/jnr.490280216","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T05:24:41Z","timestamp":1104557081000},"page":"291-298","source":"Crossref","is-referenced-by-count":26,"title":["Differential neuronal expression of c\u2010<i>fos<\/i> proto\u2010oncogene following peripheral nerve injury or chemically\u2010induced seizure"],"prefix":"10.1002","volume":"28","author":[{"given":"K. J.","family":"Jones","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Evinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"752","article-title":"Multiple pathways mediate c\u2010 fos induction by NGF in PC 12","volume":"14","author":"Armstrong RC","year":"1988","journal-title":"Neurosci Abs"},{"key":"e_1_2_1_3_1","volume-title":"Current Protocols in Molecular Biology","author":"Ausubel FM","year":"1989"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)91177-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(70)90345-8"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950070606"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(88)90033-2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(89)90150-7"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(90)90341-6"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(90)90141-E"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90993-6"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90088-1"},{"key":"e_1_2_1_14_1","first-page":"155","volume-title":"Neuronal Plasticity","author":"Grafstein B","year":"1978"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(88)90067-8"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/32.3.6198359"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90137-7"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90081-E"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90747-Y"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/328632a0"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-126-4-1849"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01611656"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)90388-0"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.21.7330"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.5.1182"},{"key":"e_1_2_1_26_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Maniatis T","year":"1982"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(77)90639-4"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902850203"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.13.4789"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3037702"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/322552a0"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(89)90096-9"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00999773"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-01-00323.1990"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(90)90068-O"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3131879"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(89)90670-8"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(89)90057-0"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1159\/000121578"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.490280216","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.490280216","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T21:38:45Z","timestamp":1698010725000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.490280216"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,2]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,2]]}},"alternative-id":["10.1002\/jnr.490280216"],"URL":"https:\/\/doi.org\/10.1002\/jnr.490280216","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,2]]}}}