{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T06:25:38Z","timestamp":1766557538055},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":4820,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1991,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mouse cerebellar granule neurons and astrocytes grown in the presence of 2 mM guanidinoethane sul\u2010fonate (GES) exhibited a progressive and rapid decrease in taurine concentration. A reduction of 20% was observed as early as 1 hr after exposure to GES and the loss of cell taurine continued until the taurine pool was reduced by about 90%. This remaining taurine persisted without further decrease even after 3 weeks of exposure to GES. Taurine reduction caused by GES was similar in both types of cells. The effect of GES was dose\u2010dependent, with significant decreases in taurine levels already detected at 100 \u03bcM. It was selective for taurine, since none of the other free amino acids were affected. Taurine depletion induced by GES was totally reversible. Intracellular taurine was not mobilized by GES. Taurine uptake in both astrocytes and granule neurons, examined at the taurine concentration present in the culture medium, was practically abolished by 2 mM GES. This approach represents an in vitro model of taurine depletion that may be useful to investigate the cell abnormalities responsible for the failure of differentiation and migration of granule cells and astrocytes observed in taurine\u2010deficient cats.<\/jats:p>","DOI":"10.1002\/jnr.490290414","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T05:50:59Z","timestamp":1104558659000},"page":"533-537","source":"Crossref","is-referenced-by-count":12,"title":["Taurine\u2010deficient cultured cerebellar astrocytes and granule neurons obtained by treatment with guanidinoethane sulfonate"],"prefix":"10.1002","volume":"29","author":[{"given":"J.","family":"Mor\u00e1n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Pasantes\u2010Morales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00964953"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1984.tb09691.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-6989(70)80048-0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1138364"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490180315"},{"key":"e_1_2_1_7_1","first-page":"77","article-title":"Taurine: Sodium\u2010dependent, high affinity transport into rat brain synaptosomes","volume":"14","author":"Hruska R","year":"1978","journal-title":"Mol Pharmacol"},{"key":"e_1_2_1_8_1","first-page":"465","article-title":"The transport of taurine in the heart and the rapid depletion of tissue taurine content by guanidinoethyl sulfonate","volume":"211","author":"Huxtable RJ","year":"1979","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1968.48.2.424"},{"key":"e_1_2_1_10_1","first-page":"239","article-title":"Effect of prolonged guani\u2010dinoethane sulfonate administration on taurine and other amino acids in rat tissues","volume":"62","author":"Marnela KM","year":"1984","journal-title":"Med Biol"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)91272-9"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490150212"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490090205"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(85)90262-7"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490110207"},{"key":"e_1_2_1_16_1","first-page":"381","volume-title":"The Biochemical Pathology of Astrocytes","author":"Schousboe A","year":"1988"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1989.tb07429.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-4448-4_9"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490130307"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-0405-8_11"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.490290414","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.490290414","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T06:33:17Z","timestamp":1698042797000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.490290414"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,8]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1991,8]]}},"alternative-id":["10.1002\/jnr.490290414"],"URL":"https:\/\/doi.org\/10.1002\/jnr.490290414","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,8]]}}}