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To determine whether these proteins are found within the same 10\u2010nm filament, light and electron microscope immunocytochemistry using peripherin and NF\u2010specific antibodies was performed on PC12 cells, nervous tissue, and isolated neural filaments from the cauda equina. Double\u2010label immunofluorescence showed that peripherin and NF\u2010L, \u2010M, and \u2010H were found in identical filamentous patterns in interphase and mitotic PC12 cells. Furthermore, expression of mutant peripherin in PC12 cells disrupted not only the peripherin network but also NF\u2010containing filaments. Immunoelectron microscopy of PC12 cell cytoskeletons showed that peripherin and NF subunit proteins were found in the same filament. In situ, in the sciatic nerve, peripherin\/NF\u2010L or peripherin\/NF\u2010M\/\u2010H double\u2010label immunofluorescence illustrates at least three types of nerve fibers: those containing NF only, those labeled predominantly for peripherin, and fibers in which peripherin and NF subunits were colocalized. Immunoelectron microscopy of filaments isolated from nerve roots comprising the sciatic nerve also showed the same three labeling patterns seen by light microscopy. Some neural IF appear to contain predominantly NF proteins or peripherin, but in others, both proteins are found within the same IF.<\/jats:p>","DOI":"10.1002\/jnr.490300110","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T07:16:13Z","timestamp":1104563773000},"page":"80-91","source":"Crossref","is-referenced-by-count":83,"title":["Some neural intermediate filaments contain both peripherin and the neurofilament proteins"],"prefix":"10.1002","volume":"30","author":[{"given":"L. 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