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With the use of a compartmented culture system it could be shown that H\u20107 exerted both local (outgrowth region) and central (ganglia) effects, the latter being more pronounced. The local effects could be due to reduction of Schwann cell proliferation by H\u20107. Immunohisto\u2010chemistry demonstrated the presence of protein kinase C in neuronal cell bodies but not in axonal processes. Proliferation of Schwann cells was accompanied by increased protein kinase C immunoreactivity at the site of injury. H\u20107 caused a selective inhibition in the incorporation of radioactive phosphate into one 74 kDa protein of both ganglia and nerve but also a more general decrease in protein labelling. The results show that protein phosphorylations, possibly mediated by protein kinase C, are involved in regeneration\u2010related mechanisms operating at both local and central levels in the adult frog sciatic sensory axons.<\/jats:p>","DOI":"10.1002\/jnr.490310308","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T18:34:48Z","timestamp":1117305288000},"page":"462-469","source":"Crossref","is-referenced-by-count":15,"title":["Effects of protein kinase inhibitors on regeneration in vitro of adult frog sciatic sensory axons"],"prefix":"10.1002","volume":"31","author":[{"given":"P. A. 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