{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T18:36:10Z","timestamp":1785004570882,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":4393,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1992,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Successful mammalian peripheral nerve regeneration is dependent on activated Schwann cells. Schwann cells facilitate neuronal regrowth through the production of tropic cell membrane molecules, neurotrophins, and extracellular matrix components. To better understand Schwann cell function in the regenerating nerve, we have designed a method of isolating proliferating adult Schwann cells from the injured rat sciatic nerve. Relying on the mitotic signal that is present after a crush injury, we can obtain sufficient numbers of dividing Schwann cells within one week of initial culture. A spontaneously immortal Schwann cell clone (iSC) was observed in and isolated from one of these primary cultures. These cells were transformed at a time of maximal Schwann cell activation in response to injury. Both the primary Schwann cells and the iSC have been characterized as Schwann cells by morphology, immunohistochemistry and gene expression. \u00a9 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490330206","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T04:23:39Z","timestamp":1104553419000},"page":"231-238","source":"Crossref","is-referenced-by-count":48,"title":["Isolation of activated adult schwann cells and a spontaneously immortal schwann cell clone"],"prefix":"10.1002","volume":"33","author":[{"given":"L. M.","family":"Bolin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. P.","family":"Iismaa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. M.","family":"Shooter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1980.00500550031001"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.5.1701"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01216702"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/266364a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(79)90048-9"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"8374","DOI":"10.1016\/S0021-9258(18)43500-4","article-title":"Purification and preliminary characterization of glial growth factor from bovine pituitary","volume":"255","author":"Brockes JP","year":"1980","journal-title":"J Biol Chem"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00591a005"},{"key":"e_1_2_1_9_1","first-page":"156","article-title":"Single step method of RNA isolation by acid guanidinium thiocyanate\u2010chloroform extraction","volume":"162","author":"Chomczymski P","year":"1987","journal-title":"Anal Biochem"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.04-08-02080.1984"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1988.7.261"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490290404"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490290204"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1523\/JNEUROSCI.08-01-00231.1988","article-title":"Nerve growth factor receptors on cultured rat Schwann cells","volume":"8","author":"DiStephano PS","year":"1988","journal-title":"J Neurosci"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1242\/dev.112.1.33","article-title":"Spontaneous Schwann cell immortalization in vitro: Short term cultured Schwann cells secrete growth inhibitory activity","volume":"112","author":"Eccleston PA","year":"1991","journal-title":"Development"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490240410"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.13.030190.000355"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(85)90085-5"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.7.2424"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.07-03-00682.1987"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.23.8735"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.109.1.91"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0387-7604(12)80331-7"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(81)90375-9"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490270320"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01189807"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.04-01-00075.1984"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.102.3.499"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90289-2"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.14.5233"},{"key":"e_1_2_1_31_1","first-page":"150","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Maniatis T","year":"1982"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1982.tb12552.x"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.2.607"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01188409"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-08-02433.1991"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-3359-0_1"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01187088"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1988.tb02938.x"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7063842"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.4.1218"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-10-03070.1986"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/325593a0"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1990.tb04888.x"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.84.3.739"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.84.3.753"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.105.2.251"},{"key":"e_1_2_1_47_1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1523\/JNEUROSCI.05-02-00379.1985","article-title":"Adhesion of axolemmal fragments to Schwann cells: A signal\u2010 and target\u2010specific process closely linked to axolemmal induction of Schwann cell mitosis","volume":"5","author":"Sobue G","year":"1985","journal-title":"J Neurosci"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1177\/37.4.2926122"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.19.6034"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(76)90355-3"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.490330206","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.490330206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T21:19:23Z","timestamp":1698095963000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.490330206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,10]]}},"alternative-id":["10.1002\/jnr.490330206"],"URL":"https:\/\/doi.org\/10.1002\/jnr.490330206","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,10]]}}}