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Cells were grown in different culture media and received FGF\u20102 (5 ng\/ml) and\/or the toxins (5 \u03bcM) at various schedules, but were consistently allowed to differentiate for 3 days prior to becoming exposed to the toxin. Survival of tyrosine hydroxylase (TH)\u2010immunoreactive cells at 7 days was only markedly impaired by MPTP, if horse serum (HS) or bovine serum albumine (BSA) were omitted from the culture medium. FGF\u20102 increased the number of TH\u2010immunoreactive cells, and this increase not diminished by MPTP under any culture condition. Uptake of <jats:sup>3<\/jats:sup>H\u2010DA was significantly reduced by MPTP in HS\u2010 and BSA\u2010containing, but not in protein\u2010less cultures. A protective effect by FGF\u20102 was only seen in the presence of BSA. MPP<jats:sup>+<\/jats:sup> caused a more pronounced reduction in <jats:sup>3<\/jats:sup>H\u2010DA uptake than MPTP, and this effect was partially reversed by the addition of FGF\u20102, unless cultures contained HS. Neurofilament protein (NF), an indirect measure for the total number of neurons present in the cultures, was not significantly reduced by MPTP or MPP<jats:sup>+<\/jats:sup> corroborating the specificity of the toxin for DAergic neurons, which constitute only a minor fraction in these cultures. In line with the wide spectrum of target neurons of FGF\u20102, this factor significantly increased NF contents under any culture condition. Quantification of the amounts of glial fibrillary acidic protein (GFAP) revealed stimulatory effects of FGF\u20102 (2.5\u2010 to 4\u2010fold) and at least 10\u2010fold higher levels in the presence as compared to the absence of HS. These data show that FGF\u20102 can protect DAergic neurons against MPTP\u2010 and MPP<jats:sup>+<\/jats:sup>\u2010mediated damage. However, the effects of the toxins as well as of FGF\u20102 are partially dependent on culture conditions. Variations in the effectiveness of toxins and FGF\u20102 are not overtly related to the total numbers of neurons or astroglial cells, but may reflect culture type\u2010dependent alterations of neuronal and glial metabolism. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490340403","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T18:47:48Z","timestamp":1117306068000},"page":"382-393","source":"Crossref","is-referenced-by-count":65,"title":["FGF\u20102\u2010mediated protection of cultured mesencephalic dopaminergic neurons against MPTP and MPP<sup>+<\/sup>: Specificity and impact of culture conditions, non\u2010dopaminergic neurons, and astroglial cells"],"prefix":"10.1002","volume":"34","author":[{"given":"D\u00f6rte","family":"Otto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Unsicker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90040-8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2887034"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(80)90202-5"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)91293-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1523\/JNEUROSCI.08-03-00733.1988","article-title":"Development and survival of neurons in dissociated fetal mesencephalic serum\u2010free cell cultures. 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