{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T21:29:50Z","timestamp":1789853390687,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":4120,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1993,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Semliki Forest virus expression vector (Liljestr\u00f6m and Garoff: Bio\/Technology 9:1356\u20101361, 1991) was tested in cultured rat hippocampal neurons using two Madin\u2010Darby canine kidney (MDCK) cell membrane\u2010associated proteins as reporters: rab8, a small GTP ase involved in post\u2010Golgi vesicle transport, and VIP21, an integral membrane protein of caveolae, trans\u2010Golgi network, and post\u2010Golgi vesicles. Expression of the c\u2010<jats:italic>myc<\/jats:italic> epitope\u2010tagged proteins was visualized by immunofluorescence microscopy. The proteins were first detected in neurons after 3\u20134 hr infection by the recombinant viruses. The infection efficiency on neurons was high: after 6 hr infection at a multiplicity of one, 50\u201360% of the cells expressed the reporter proteins. The neurons tolerated the in fection well up to 8 hr. Their polarized organization was not disturbed, as judged from morphology and from distribution of the dendritic MAP2 and axonal synaptophysin marker proteins. The Semliki Forest virus vector thus seems suitable for short\u2010term expression of proteins in cultured neurons. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490350412","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T01:49:37Z","timestamp":1104544177000},"page":"445-451","source":"Crossref","is-referenced-by-count":66,"title":["Expression of heterologous proteins in cultured rat hippocampal neurons using the semliki forest virus vector"],"prefix":"10.1002","volume":"35","author":[{"given":"V. 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