{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T08:46:32Z","timestamp":1698223592328},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":4028,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1993,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ionic mechanism of the effect of extracellularly ejected calcitonin (CT) on the membrane of identified neurons R9 and R10 of <jats:italic>Aplysia<\/jats:italic> was investigated with voltage\u2010clamp, micropressure ejection, and ion substitution techniques. Micropressure\u2010ejected CT caused a marked hyperpolarization in the unclamped neuron. Heat\u2010inactivated CT was without effect. Clamping the same neuron at its resting potential level (\u221260mV) and re\u2010ejecting CT with the same dose produced a slow outward current (I<jats:sub>o<\/jats:sub>(CT), 30\u201340 sec in duration, 4\u20136 nA in amplitude) associated with a decrease in input membrane conductance. I<jats:sub>o<\/jats:sub> (CT) was decreased by depolarization and increased by hyperpolarization. The extrapolated reversal potential of I<jats:sub>o<\/jats:sub> was approximately +10mV. I<jats:sub>o<\/jats:sub> (CT) was sensitive to changes in the external Na<jats:sup>+<\/jats:sup> concentration but not to changes in K<jats:sup>+<\/jats:sup>, Ca<jats:sup>2+<\/jats:sup>, and CI<jats:sup>\u2212<\/jats:sup> concentrations. Micropressure\u2010ejected forskolin produced a slow outward current, which, like the current to CT, was associated with a decrease in input membrane conductance, and was sensitive to changes in the external Na<jats:sup>+<\/jats:sup> concentration. I<jats:sub>o<\/jats:sub> (CT) was prolonged by bath\u2010applied isobutylmethylxanthine (IBMX) but was not affected by I\u2010oleoyl\u20102\u2010acetylglycerol (OAG) and calphostin C. Neither superfusion of the neuron with nordihydroguaiaretic acid (NDGA) nor superfusion with indomethacin caused any changes in I<jats:sub>o<\/jats:sub>(CT). These results suggest that extracellular CT can induce a slow outward current associated with a decrease in Na<jats:sup>+<\/jats:sup> conductance, mediated by a receptor\u2010controlled increase in intracellular cyclic adenosine 3\u2032, 5\u2032\u2010monophosphate. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490360210","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T01:52:10Z","timestamp":1104544330000},"page":"200-208","source":"Crossref","is-referenced-by-count":3,"title":["Calcitonin induces a decreased Na<sup>+<\/sup> conductance in identified neurons of <i>Aplysia<\/i>"],"prefix":"10.1002","volume":"36","author":[{"given":"M.","family":"Sawada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ichinose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Sasayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/298240a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.8.2538"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-122-4-1218"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.18.7094"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.6.4.551"},{"key":"e_1_2_1_7_1","first-page":"431","volume-title":"Handbook of Physiology","author":"Copp DH","year":"1976"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/313313a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/347182a0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.3.815"},{"key":"e_1_2_1_11_1","first-page":"133","article-title":"Membrane potential changes, cAMP stimulation and contraction and contraction in osteoblast\u2010like UMR 106 cells in response to calcitonin and parathyroid hormone","volume":"4","author":"Ferrier J","year":"1988","journal-title":"Bone Miner"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-57-6-1314"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(81)80030-5"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1967.30.6.1288"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.493987"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(81)90032-9"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2432663"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-94-1-241"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.130.1.381"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","unstructured":"IshikawaS SasayamaY OguroC(1991): Calcitonin\u2010immunoreactive cells present in the abdominal ganglion of the seahare Aplysia kurodai. 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