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In situ hybridization and immunocytochemical studies were performed to identify the structures expressing GDN\/PN\u20101 in the developing and adult rat brain. In contrast to a transient widespreat expression during pre\u2010and postnatal development, some brain structures constitutively express GDN\/PN\u20101. These include the olfactory nerve layer of the olfactory bulb, basal forebrain, striatum, pyramidal neurons of layer V in the cortex, thalamic nuclei, pars compacta of the substantia nigra, inferior and superior colliculi, and deep cerebellar nuclei. All of these parts, excluding the olfactory nerve layer, are characterized by a high neuronal cell density. Neurons in these regions were immunoreactive for GDN\/PN\u20101. Furthermore GDN\/PN\u20101 expression in cell lines showed that the active protein was synthesized and secreted from B104 but not from NB2a neuroblastoma cells. Although GDN\/PN\u20101 has only been reported to be synthesized by glia, the results presented here demonstrate that in addition, a subset of neurons express this protease inhibitor. \u00a9 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490370211","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T01:55:33Z","timestamp":1104544533000},"page":"256-270","source":"Crossref","is-referenced-by-count":56,"title":["Glia\u2010derived nexin\/protease nexin\u20101 is expressed by a subset of neurons in the rat brain"],"prefix":"10.1002","volume":"37","author":[{"given":"E.","family":"Reinhard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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