{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T12:48:38Z","timestamp":1793450918576,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":3877,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1994,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two peripheral myelin protein <jats:italic>PMP22<\/jats:italic> transcripts, <jats:italic>CD25<\/jats:italic> and <jats:italic>Sr13<\/jats:italic>, have been identified by Northern blot and RNA\u2010polymerase chain reaction (PCR) methods in rat. The <jats:italic>CD25<\/jats:italic> and <jats:italic>SR13<\/jats:italic> mRNA species (each approximately 1.8 kb in size) differ significantly in their 5\u2032\u2010untranslated region (5\u2032\u2010UTR) sequences but encode the same protein. While <jats:italic>CD25<\/jats:italic> mRNA is largely confined to the peripheral nervous system, the <jats:italic>Sr13<\/jats:italic> transcript is more ubiquitously expressed in rat tissues. Both transcripts are differentially expressed during postnatal sciatic nerve development, while <jats:italic>CD25<\/jats:italic> expression steadily increases from low levels in neonates up to a maximum at postnatal day 14, <jats:italic>SR13<\/jats:italic> mRNA levels are elevated at birth but decrease throughout adulthood. <jats:italic>CD25<\/jats:italic> and <jats:italic>Sr13<\/jats:italic> transcripts are expressed at very low constant levels in developing and adult brain. In degenerating and regenerating segments of injured peripheral nerve changes in <jats:italic>CD25<\/jats:italic> mRNA levels clarly resemble the expression pattern of other myelin genes, whereas expression of <jats:italic>SR13<\/jats:italic> is inversely correlated with the time course of Schwann cell proliferation. In cultured rat meningeal fibroblast <jats:italic>SR13<\/jats:italic> mRNA expression is strictly growth arrest\u2010specific and independent of forskolin. On the other hand, regulation of <jats:italic>CD25<\/jats:italic> mRNA levels in these cells is more complex with respect to interfering effects of serum and forskolin. In cultured Schwann cells neither <jats:italic>CD25<\/jats:italic> nor <jats:italic>SR13<\/jats:italic> expression is growth arrest\u2010specific. However, both transcript levels are consistently enhanced by forskolin under all conditions of cell growth tested. Expression of <jats:italic>CD25<\/jats:italic> (but not <jats:italic>SR13<\/jats:italic>) depends on high Schwann cell density. Our results substantiate the hypothesis that <jats:italic>PMP22<\/jats:italic> serves two biological functions, one related to cell growth (<jats:italic>SR13<\/jats:italic>) and another to myelination (<jats:italic>CD25<\/jats:italic>). \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490370412","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T01:57:20Z","timestamp":1104544640000},"page":"529-537","source":"Crossref","is-referenced-by-count":71,"title":["Differential expression of two mRNA species indicates a dual function of peripheral myelin protein <i>PMP22<\/i> in cell growth and myelination"],"prefix":"10.1002","volume":"37","author":[{"given":"F.","family":"Bosse","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Zoidl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Wilms","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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