{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:32:22Z","timestamp":1771003942548,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":3771,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1994,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Total deficiency of hypoxanthine phosphoribosyl\u2010transferase (HPRT) in humans causes the neurological disorder Lesch\u2010Nyhan syndrome. The HPRT gene is expressed at basal levels in all tissues but at higher levels in the brain, the relevance and mechanism of which is unknown. To determine if <jats:italic>cis<\/jats:italic>\u2010acting DNA elements play a role in the tissue\u2010differential pattern of expression, we generated transgenic mice carrying different sequences of the human HPRT (hHPRT) promoter fused to the bacterial <jats:italic>lacZ<\/jats:italic> gene. We show that a 1.6 kb fragment of the hHPRT promoter contains essential information to direct \u03b2\u2010galactosidase expression preferentially to the basal ganglia, cerebral cortex, hippocampus, and several other areas of the forebrain. At least two elements within the 1.6 kb fragment appear to be required for neuronal expression. A 182 bp element (hHPRT\u2010NE) represents one of those sequences and is involved not only in confering neuronal specificity but also in repressing transgene expression in non\u2010neuronal tissues. These studies provide molecular insight into the mechanism of increased HPRT expression in the brain. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490380304","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T01:58:50Z","timestamp":1104544730000},"page":"259-267","source":"Crossref","is-referenced-by-count":16,"title":["5\u2032\u2010flanking sequences of the human HPRT gene direct neuronal expression in the brain of transgenic mice"],"prefix":"10.1002","volume":"38","author":[{"given":"D. E.","family":"Rinc\u00f3n\u2010Limas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. S.","family":"Geske","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u2010J.","family":"Xue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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