{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T05:48:05Z","timestamp":1791006485984,"version":"4.1.0"},"reference-count":65,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":3588,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1994,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adult dorsal root ganglion (DRG) cells are capable of neurite outgrowth in vivo and in vitro after axotomy. We have investigated, in cultured adult rat DRG cells, the relative influence of nerve growth factor (NGF) or a prior peripheral nerve lesion on the capacity of these neurons to produce neurites. Since there is evidence suggesting that the growth\u2010associated protein GAP\u201043 may play a crucial role in axon elongation during development and regeneration, we have also compared the effect of these treatments on GAP\u201043 mRNA expression. NGF increased the early neurite outgrowth in a subpopulation of DRG cells. This effect was substantially less, however, than that resulting from preaxotomy, which initiated an early and profuse neurite outgrowth in almost all cells. No difference in the expression of GAP\u201043 mRNA was found between neurons grown in the presence or absence of NGF over 1 week of culture, in spite of the increased growth produced by NGF. In contrast, cultures of neurons that had been preaxotomized showed substantial increase in GAP\u201043 mRNA and NGF had, as expected, a significant effect on substance P mRNA levels. Two forms of growth may be present in adult DRG neurons: an NGF\u2010independent, peripheral nerve injury\u2010provoked growth associated with substantial GAP\u201043 upregulation, and an NGF\u2010dependent growth that may underlie branching or sprouting of NGF\u2010sensitive neurons, but which is not associated with increased levels of GAP\u201043 mRNA. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490390603","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T02:04:19Z","timestamp":1104545059000},"page":"634-645","source":"Crossref","is-referenced-by-count":61,"title":["Neurite outgrowth and GAP\u201043 mRNA expression in cultured adult rat dorsal root ganglion neurons: Effects of NGF or prior peripheral axotomy"],"prefix":"10.1002","volume":"39","author":[{"given":"M.","family":"Hu\u2010Tsai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Winter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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