{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:48:04Z","timestamp":1790020084294,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":3406,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1995,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of the present study was to characterize GnRH\u2010like substance(s) in the rat pineal gland using a monoclonal antibody, LRH13, as a probe. The epitope of LRH13 is between 2nd and 5th amino acid residues of the mammalian GnRH, and its immunological characters were previously defined by us. LRH13 could show strong immunological signal on the rat pineal gland. Immunoblot after SDS\u2010PAGE of the pineal gland preparations showed a LRH13 immunoreactive band with apparent mol wt 52 kiloDalton (kD), which is much bigger than that of hypothalamic GnRH precursor (10 kD). The 52 kD protein, however, was detected from insoluble fraction of the pineal homogenate and liberated from the fraction by Triton X\u2010100 (2%) treatment. On the other hand, NaCl (140 mM and 500 mM) or EDTA (10 mM) treatment failed to liberate. Two\u2010dimensional gel electrophoresis showed that the 52 kD protein is a mixture of two proteins with different isoelectric points (pI approximately 6.8 and 7.0). Both proteins showed identical patterns of peptide mapping by V8 protease digestion, and they might be originated from the same peptide. These results suggest that the rat pineal GnRH\u2010immunoreactive substance has a unique property as a membrane associate protein. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490410311","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T02:10:37Z","timestamp":1104545437000},"page":"386-393","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of gonadotropin\u2010releasing hormone (GnRH)\u2010immunoreactive protein in the rat pineal gland"],"prefix":"10.1002","volume":"41","author":[{"given":"M. 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