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Screening of 5,000 randomly selected colonies yielded 24 distinct clones that were regulated following nerve injury. Fifteen of the differentially expressed sequences could be classified as induced, whereas 9 sequences appeared to be repressed at 1 week postcrush. Sequencing and computer\u2010assisted sequence comparison revealed 3 classes of regulated cDNA clones representing (1) novel gene sequences (8 clones) including 3 transcripts containing a repetitive \u201cbrain identifier\u201d (ID) element; (2) identified genes (7 clones) with previously undetected expression in the peripheral nervous system (PNS), such as apolipoprotein D, peripheral myelin protein 22kD (PMP22), SPARC (secreted protein, acidic and rich in cysteine), sulfated glycoprotein SGP\u20101, apoferritin, decorin, and X16\/SRp20; and (3) identified genes (9 clones) with known expression in the PNS including, e.g., the myelin protein P<jats:sub>0<\/jats:sub>, \u03b3\u2010actin, vimentin, a\u2010tubulin, chargerin II, and cytochrome c\u2010oxidase subunit I. Northern blot and polymerase chain reaction analyses with RNA from crushed and transected nerve demonstrated that sequences with related function, like the group of myelin genes, cytoskeleton genes, genes involved in RNA processing and translation, in lipid transport or energy metabolism showed closely related temporal patterns of expression during nerve degeneration and regeneration. Finally, we compared the differentially expressed genes identified at 7 days after crush injury (this investigation) with the regulated sequences isolated previously by De Leon et al. (J Neurosci Res 29:437\u2010488, 1991) from a 3 day postcrush sciatic nerve cDNA library. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490420203","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T02:13:56Z","timestamp":1104545636000},"page":"159-171","source":"Crossref","is-referenced-by-count":69,"title":["Differentially expressed genes after peripheral nerve injury"],"prefix":"10.1002","volume":"42","author":[{"given":"C.","family":"Gillen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Gleichmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Spreyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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