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The extent of protein degradation was determined by SDS\u2010PAGE and densitometric analysis. Various proteinase inhibitors were used to determine to what extent protein degradation was mediated by calpain and\/or other enzymes. Lysosomal and serine proteinase inhibitors inhibited 20\u201040% of the myelindegradative activity found in the incubation media of cell lines, whereas the calcium chelator (EGTA), the calpain\u2010specific inhibitor (calpastatin), and a monoclonal antibody to m calpain blocked myelin degradation by 60\u201080%. 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