{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:20:55Z","timestamp":1791220855381,"version":"4.1.0"},"reference-count":98,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,11,19]],"date-time":"2004-11-19T00:00:00Z","timestamp":1100822400000},"content-version":"vor","delay-in-days":3245,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1996,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We describe here a novel monoclonal antibody (mab INTRODUCTION H6) which recognizes CD9, an integral cell surface constituent previously described in cells of the hematopoietic lineage and involved in the aggregation of platelets. Mab H6 was raised against membranes of immature mouse astrocytes and reacted with a protein of 25\u201327 kD in detergent extracts of adult mouse brain membranes. Sequence analysis of the N\u2010terminal amino acids revealed an identity of 96% with CD9 from mouse kidney. CD9 was localized in the central and peripheral mouse nervous systems: in the spinal cord of 11\u2010day\u2010old mouse embryos, CD9 was strongly expressed in the floor and roof plates. In the adult mouse sciatic nerve, myelin sheaths were highly CD9immunoreactive. Mab H6 reacted with the cell surfaces of both glial cells and neurons in culture and inhibited migration of neuronal cell bodies, neurite fasciculation and outgrowth of astrocytic processes from cerebellar microexplants. Neurite outgrowth from isolated small cerebellar neurons was increased in the presence of mab H6 on substrate\u2010coated laminin, but not on substrate\u2010coated poly\u2010L\u2010lysine. Addition of mab H6 elicited an increase in intracellular Ca<jats:sup>2+<\/jats:sup> concentration in these cells on substrate\u2010coated laminin. Immunoprecipitates of CD9 from cultured mouse neuroblastoma N2A cells contained the \u03b16\/\u03b21 integrin. Moreover, preparations of CD9 immunoaffinity\u2010purified from adult mouse brain using a mab H6 column contained the neural adhesion molecule L1, but not other neural adhesion molecules. CD9 bound to L1, but not to NCAM or MAG. Both the \u03b16\/\u03b21 integrin and L1 could be induced to coredistribute with CD9 on the surface of cultured neuroblastoma N2A cells. The combined observations suggest that CD9 can associate with L1 and \u03b16\/\u03b21 integrin to influence neural cell interactions in vitro. \u00a9 1996 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jnr.490430103","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T02:17:00Z","timestamp":1104545820000},"page":"12-31","source":"Crossref","is-referenced-by-count":87,"title":["CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the \u03b16\/\u03b21 integrin and the neural adhesion molecule L1"],"prefix":"10.1002","volume":"43","author":[{"given":"C.","family":"Schmidt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"K\u00fcnemund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. S.","family":"Wintergerst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Schmitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Schachner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,11,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490120105"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.6.1587"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"4764","DOI":"10.1523\/JNEUROSCI.13-11-04764.1993","article-title":"Several extracellular domains of the neural cell adhesion molecule L1 are involved in neurite outgrowth and cell body adhesion","volume":"13","author":"Appel F","year":"1993","journal-title":"J Neurosci"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","unstructured":"AppelF HolmJ ConscienceJ\u2010F von BohlenJ FaissnerAPJ SchachnerM(1995): Identification of the border between fibronectin type III homologous repeats 2 and 3 of the neural cell adhesion molecule L1 as a neurite outgrowth promoting and signal transducing domain.J Neurobiol in press.","DOI":"10.1002\/neu.480280304"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(83)81027-8"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0021-9258(18)52410-8","article-title":"Molecular cloning of the CD9 antigen","volume":"266","author":"Boucheix C","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90172-N"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(08)60210-0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/ncmn.1993.1024"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490370407"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.3.843"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-4388(92)90024-F"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90569-K"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.09.040191.000331"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02590.x"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1002\/j.1460-2075.1984.tb01876.x","article-title":"The neural cell adhesion molecule L1 is distinct from the N\u2010CAM related group of surface antigens BSP\u20102 and D2","volume":"3","author":"Faissner A","year":"1984","journal-title":"EMBO J"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1985.tb04052.x"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01740775"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(82)90079-9"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(82)90333-0"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-02-00605.1986"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.118.1.177"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90223-2"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.4.8.1970792"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90062-5"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(92)90099-X"},{"key":"e_1_2_1_28_1","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1182\/blood.V78.7.1753.1753","article-title":"Platelet activation by immobilized monoclonal antibody: Evidence for a CD9 proximal signal","volume":"78","author":"Griffith L","year":"1991","journal-title":"Blood"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90068-3"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.6.2175"},{"key":"e_1_2_1_31_1","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1182\/blood.V72.1.224.224","article-title":"Exposure of platelet fibrinogen receptors by a monoclonal antibody to CD9 antigen","volume":"1","author":"Hato T","year":"1988","journal-title":"Blood"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90178-5"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(82)90164-6"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)80988-F"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.121.6.1409"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90115-S"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90472-O"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.114.5.1069"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.6.1249"},{"key":"e_1_2_1_40_1","doi-asserted-by":"crossref","first-page":"3815","DOI":"10.1016\/S0021-9258(19)39667-X","article-title":"The activation of human platelets mediated by antihuman platelet p24\/CD9 monoclonal antibodies","volume":"265","author":"Jennings LK","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.1.193"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.1.209"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1995.tb01087.x"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-06-02495.1993"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(94)90047-7"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/316728a0"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90148-K"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(92)90144-Z"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.1.213"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.21.7753"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(80)90122-0"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.480230108"},{"key":"e_1_2_1_54_1","doi-asserted-by":"crossref","first-page":"10638","DOI":"10.1016\/S0021-9258(18)99271-9","article-title":"cDNA cloning and expression of platelet p24\/CD9","volume":"266","author":"Lanza F","year":"1991","journal-title":"J. Biol Chem."},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)98728-4"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80064-L"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(93)90049-X"},{"key":"e_1_2_1_58_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.5.1765"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.6.2439"},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01257247"},{"key":"e_1_2_1_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a123866"},{"key":"e_1_2_1_62_1","first-page":"2768","article-title":"CD9\u2010regulated adhesion","volume":"152","author":"Masellis\u2010Smith A","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_1_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150030104"},{"key":"e_1_2_1_64_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.3.1177"},{"key":"e_1_2_1_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/362075a0"},{"key":"e_1_2_1_66_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1948065"},{"key":"e_1_2_1_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.8.2444"},{"key":"e_1_2_1_68_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.4.1893"},{"key":"e_1_2_1_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490300315"},{"key":"e_1_2_1_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb01828.x"},{"key":"e_1_2_1_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb01753.x"},{"key":"e_1_2_1_72_1","doi-asserted-by":"publisher","DOI":"10.1159\/000111910"},{"key":"e_1_2_1_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(93)90162-H"},{"key":"e_1_2_1_74_1","doi-asserted-by":"publisher","DOI":"10.3109\/15419069309095682"},{"key":"e_1_2_1_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1989.tb08540.x"},{"key":"e_1_2_1_76_1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1182\/blood.V67.1.50.50","article-title":"A monoclonal antibody reactive with human eosinophils","volume":"67","author":"Saito H","year":"1986","journal-title":"Blood"},{"key":"e_1_2_1_77_1","unstructured":"SchmidtC K\u00fcnemundV FalchettoR SchachnerM SchmitzB(1992): CD9 in the mouse nervous system.Int Soc Cell Biol Abstr12.2\u2013141."},{"key":"e_1_2_1_78_1","first-page":"97.1","article-title":"CD9 of mouse brain is implicated in neurite outgrowth and cell migration and is associated with L1 and \u03b16\u03b21 integrin","volume":"20","author":"Schmidt C","year":"1994","journal-title":"Am Soc Neurosci."},{"key":"e_1_2_1_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(81)90022-9"},{"key":"e_1_2_1_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90111-6"},{"key":"e_1_2_1_81_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.123.4.1017"},{"key":"e_1_2_1_82_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.6.2167"},{"key":"e_1_2_1_83_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1991.tb00849.x"},{"key":"e_1_2_1_84_1","doi-asserted-by":"crossref","first-page":"12289","DOI":"10.1016\/S0021-9258(18)63856-6","article-title":"Evidence that monoclonal antibodies against CD9 antigen induce specific association between CD9 and the platelet glycoprotein IIb\u2010IIIa complex","volume":"264","author":"Slupsky JR","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_1_85_1","doi-asserted-by":"publisher","DOI":"10.1177\/34.8.2426332"},{"key":"e_1_2_1_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)61123-8"},{"key":"e_1_2_1_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(91)90428-W"},{"key":"e_1_2_1_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90081-P"},{"key":"e_1_2_1_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(87)90238-1"},{"key":"e_1_2_1_90_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2006419"},{"key":"e_1_2_1_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(86)90176-X"},{"key":"e_1_2_1_92_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001970203"},{"key":"e_1_2_1_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90207-3"},{"key":"e_1_2_1_94_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_95_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1992.tb00116.x"},{"key":"e_1_2_1_96_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1990.tb15569.x"},{"key":"e_1_2_1_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(88)90101-0"},{"key":"e_1_2_1_98_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.4.883"},{"key":"e_1_2_1_99_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90147-J"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.490430103","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.490430103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T06:44:48Z","timestamp":1698389088000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.490430103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,1]]},"references-count":98,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,1]]}},"alternative-id":["10.1002\/jnr.490430103"],"URL":"https:\/\/doi.org\/10.1002\/jnr.490430103","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,1]]}}}