{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T05:38:31Z","timestamp":1792733911629,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P50AA017823"],"award-info":[{"award-number":["P50AA017823"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Adolescent binge alcohol alters late\u2010developing brain regions and increases the risk for long\u2010term reward, cognitive, and emotional dysregulation. Adolescent intermittent ethanol (AIE) exposure in rodents produces similar behavioral alterations into adulthood, but the effects of acute high\u2010dose ethanol on patterns of neuronal activation in late developing brain regions remain poorly understood. In the current study, adolescent male and female cFos\u2010LacZ rats were acutely challenged with water or 4\u2009g\/kg ethanol intragastrically (IG) on postnatal day (P) 25 or P45, early\u2010 and late\u2010adolescence, with brains collected 1\u2009h later. P45 rats with a history of water or AIE (10 exposures every other day from P25 to P45) were included as a chronic exposure condition. \u03b2\u2010galactosidase expression was used as a proxy for Fos neuronal activation. Acute IG gavage increased neuronal activation in multiple brain regions irrespective of water or ethanol challenge, and increases were attenuated by adolescent chronic exposure. Increased neuronal activation was evident only in the central amygdala (CeA) after acute ethanol challenge, with this effect being attenuated by AIE. Functional network mapping revealed age\u2010, sex\u2010, and exposure\u2010related differences: water\u2010exposed adolescent males had salient network hub regions, while control females had age\u2010related transitions from salient to executive control network regions. Ethanol disrupted these trajectories. In males, acute ethanol reduced functional network connectivity, with chronic ethanol decentralizing hubs. In females, ethanol enhanced connectivity centered on the nucleus accumbens. These findings highlight the CeA as an ethanol\u2010sensitive region across sexes, but reveal sex differences in adolescent ethanol exposure on functional networks.<\/jats:p>","DOI":"10.1002\/jnr.70163","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T03:37:11Z","timestamp":1791776231000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of Age and Prior History of Ethanol Exposure on Ethanol\u2010Induced Patterns of Neuronal Activation in\n                    <scp>cFos<\/scp>\n                    \u2010\n                    <scp>LacZ<\/scp>\n                    Adolescent Male and Female Rats"],"prefix":"10.1002","volume":"104","author":[{"given":"Harper J.","family":"Coleman","sequence":"first","affiliation":[{"name":"Department of Psychology Binghamton University  Binghamton New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3621-2772","authenticated-orcid":false,"given":"Devon T.","family":"Applegate","sequence":"additional","affiliation":[{"name":"Department of Psychology Binghamton University  Binghamton New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3451-6115","authenticated-orcid":false,"given":"Kimberly M.","family":"Papastrat","sequence":"additional","affiliation":[{"name":"Department of Psychology Binghamton University  Binghamton New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor T.","family":"Towner","sequence":"additional","affiliation":[{"name":"Department of Psychology Binghamton University  Binghamton New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena I.","family":"Varlinskaya","sequence":"additional","affiliation":[{"name":"Department of Psychology Binghamton University  Binghamton New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2914-7706","authenticated-orcid":false,"given":"David F.","family":"Werner","sequence":"additional","affiliation":[{"name":"Department of Psychology Binghamton University  Binghamton New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.psych.60.110707.163514"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ynstr.2020.100226"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20102952(92)90214\u20104"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijdevneu.2015.03.008"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2007\u20102243D"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dr.2007.08.003"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/brain.2015.0379"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.35946\/arcr.v39.1.02"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0006\u20108993(99)02468\u20103"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyg.2017.01111"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530\u20100277.2008.00806.x"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.12298"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2008\u20100027"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2009.03.025"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889\u2010019\u20107099\u20100"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2024.1498201"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.122653799"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222111733"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nlm.2017.11.016"},{"issue":"3","key":"e_1_2_12_21_1","first-page":"329","article-title":"A Spoonful of Sugar Helps the Medicine Go Down: A Novel Technique to Improve Oral Gavage in Mice","volume":"49","author":"Hoggatt A. F.","year":"2010","journal-title":"Journal of the American Association for Laboratory Animal Science"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593\u2010021\u201000828\u20102"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010clinpsy\u2010050212\u2010185610"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035\u2010025\u201005203\u2010x"},{"issue":"5","key":"e_1_2_12_25_1","first-page":"369","article-title":"Long\u2010Term Repeated Daily Use of Intragastric Gavage Hinders Induction of Oral Tolerance to Ovalbumin in Mice","volume":"64","author":"Kinder J. M.","year":"2014","journal-title":"Comparative Medicine"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1998.tb03677.x"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2020.172906"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380\u2010025\u201003037\u20106"},{"issue":"4","key":"e_1_2_12_29_1","first-page":"163","article-title":"Differences Between Male and Female Rats in Alcohol Drinking, Negative Affects and Neuronal Activity After Acute and Prolonged Abstinence","volume":"11","author":"Li J.","year":"2019","journal-title":"International Journal of Physiology, Pathophysiology and Pharmacology"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2015.02.014"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3714\u201004.2004"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0006\u20108993(97)00308\u20109"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2017.03.037"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20104159.2008.05835.x"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.113.054635"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2009.11.007"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1138\u201017.2019"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jadohealth.2024.02.014"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2016.07.026"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530\u20100277.2011.01527.x"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2024.115118"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.841657"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2023.109663"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2021.768294"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nicl.2020.102529"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.70163","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jnr.70163","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.70163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:57:49Z","timestamp":1792731469000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.70163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":44,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/jnr.70163"],"URL":"https:\/\/doi.org\/10.1002\/jnr.70163","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2025-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70163"}}