{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T18:24:12Z","timestamp":1788719052708,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,4,5]],"date-time":"2005-04-05T00:00:00Z","timestamp":1112659200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ethanolic extracts and some fractions from 10 Indian medicinal plants, known for antibacterial activity, were investigated for their ability to inhibit clinical isolates of \u03b2\u2010lactamase producing methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) and methicillin\u2010sensitive <jats:italic>S. aureus<\/jats:italic> (MSSA). Synergistic interaction of plant extracts with certain antibiotics was also evaluated. The MRSA test strains were found to be multi\u2010drug resistant and also exhibited high level of resistance to common \u03b2\u2010lactam antibiotics. These strains produced \u03b2\u2010lactamases, which hydrolyze one or other \u03b2\u2010lactam antibiotics, tested. The extract of the plants from <jats:italic>Camellia sinensis<\/jats:italic> (leaves), <jats:italic>Delonix regia<\/jats:italic> (flowers), <jats:italic>Holarrhena antidysenterica<\/jats:italic> (bark), <jats:italic>Lawsonia inermis<\/jats:italic> (leaves), <jats:italic>Punica granatum<\/jats:italic> (rind), <jats:italic>Terminalia chebula<\/jats:italic> (fruits) and <jats:italic>Terminalia belerica<\/jats:italic> (fruits) showed a broad\u2010spectrum of antibacterial activity with an inhibition zone size of 11 mm to 27 mm, against all the test bacteria. The extracts from the leaves of <jats:italic>Ocimum sanctum<\/jats:italic> showed better activity against the three MRSA strains. On the other hand, extracts from <jats:italic>Allium sativum<\/jats:italic> (bulb) and <jats:italic>Citrus sinensis<\/jats:italic> (rind) exhibited little or no activity, against MRSA strains. The antibacterial potency of crude extracts was determined in terms of minimum inhibitory concentration (MIC) by the tube dilution method. MIC values, of the plant extracts, ranged from 1.3 to 8.2 mg\/ml, against the test bacteria. Further, the extracts from <jats:italic>Punica granatum<\/jats:italic> and <jats:italic>Delonix regia<\/jats:italic> were fractionated in benzene, acetone and methanol. Antibacterial activity was observed in acetone as well as in the methanol fractions<jats:italic>. In vitro<\/jats:italic> synergistic interaction of crude extracts from <jats:italic>Camellia sinensis<\/jats:italic>, <jats:italic>Lawsonia inermis<\/jats:italic>, <jats:italic>Punica granatum<\/jats:italic>, <jats:italic>Terminalia chebula<\/jats:italic> and <jats:italic>Terminalia belerica<\/jats:italic> was detected with tetracycline. Moreover, the extract from <jats:italic>Camellia sinensis<\/jats:italic> also showed synergism with ampicillin.<\/jats:p><jats:p>TLC of the above extracts revealed the presence of major phytocompounds, like alkaloids, glycosides, flavonoids, phenols and saponins. TLC\u2010bioautography indicated phenols and flavonoids as major active compounds. (\u00a9 2005 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jobm.200410355","type":"journal-article","created":{"date-parts":[[2005,4,5]],"date-time":"2005-04-05T14:46:56Z","timestamp":1112712416000},"page":"106-114","source":"Crossref","is-referenced-by-count":105,"title":["Effect of certain bioactive plant extracts on clinical isolates of \u03b2\u2010lactamase producing methicillin resistant <i>Staphylococcus aureus<\/i>"],"prefix":"10.1002","volume":"45","author":[{"given":"Farrukh","family":"Aqil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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