{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T23:30:23Z","timestamp":1788219023696,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,8,21]],"date-time":"2008-08-21T00:00:00Z","timestamp":1219276800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The presence of the <jats:italic>ica<\/jats:italic> loci and adhesins genes in clinical <jats:italic>Staphylococcus aureus<\/jats:italic> strains were considered important factors of virulence. In this study, 46 strains of <jats:italic>Staphylococcus aureus<\/jats:italic> were isolated from auricular infection, and were investigated for slime production using Congo Red Agar method (CRA). In order to detect the adhesins genes (<jats:italic>ica<\/jats:italic> A, <jats:italic>ica<\/jats:italic> D, <jats:italic>fnb<\/jats:italic> A, <jats:italic>cna<\/jats:italic>, <jats:italic>Clf<\/jats:italic> A) Polymerase Chain Reaction was used. Qualitative biofilm production of <jats:italic>S. aureus<\/jats:italic> using CRA plates revealed that 56.5% of strains were slime producers. In addition 78.26% of strains were <jats:italic>ica<\/jats:italic> A and<jats:italic> ica<\/jats:italic> D positive. While the <jats:italic>fnbA<\/jats:italic> gene was present in 76.1% of isolated strains. Furthermore, 56.5% of strains have the <jats:italic>cna<\/jats:italic> gene and 30.4% were <jats:italic>clfA<\/jats:italic> positives. Overall this study confirms the presence of <jats:italic>fnb<\/jats:italic> A and<jats:italic> ica<\/jats:italic> A\/<jats:italic>ica<\/jats:italic> D genes in the majority of studies <jats:italic>S. aureus<\/jats:italic> strains isolated from Staphylococcal sepsis. (\u00a9 2008 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jobm.200700289","type":"journal-article","created":{"date-parts":[[2008,8,21]],"date-time":"2008-08-21T15:16:11Z","timestamp":1219331771000},"page":"308-314","source":"Crossref","is-referenced-by-count":40,"title":["Detection by PCR of adhesins genes and slime production in clinical <i>Staphylococcus aureus<\/i>"],"prefix":"10.1002","volume":"48","author":[{"given":"Tarek","family":"Zmantar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamel","family":"Chaieb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9la","family":"Makni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanene","family":"Miladi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fethi Ben","family":"Abdallah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kacem","family":"Mahdouani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amina","family":"Bakhrouf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/374554"},{"key":"e_1_2_1_3_2","unstructured":"Waldvogel F.A. 1995.Staphylococcus aureus(including toxic shock syndrome). 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