{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T17:05:02Z","timestamp":1780074302124,"version":"3.54.0"},"reference-count":44,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2010,8,30]],"date-time":"2010-08-30T00:00:00Z","timestamp":1283126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study diarrheagenic and uropathogenic <jats:italic>Escherichia coli<\/jats:italic> (UPEC) strains were comparatively characterized according to serotype, hemolytic activity, protein polymorphism among housekeeping enzymes, phylogenetic group and urovirulence genes. Intra\u2010serogroup\/serotype variations were observed. Hemolytic activity was detected in 100%, 93%, 67% and 39% of UPEC, EAEC, EPEC and ETEC strains, respectively. The alpha\u2010hemolytic phenotype was observed in all pathogenic groups while beta\u2010hemolytic phenotype was less frequent. PCR phylotyping revealed higher prevalence of diarrheagenic <jats:italic>E. coli<\/jats:italic> in groups A and D while uropathogenic strains were mainly found in subgroup B2. Amplification assays revealed that 74%, 45% and 22% of UPEC, EAEC and EPEC strains, respectively, carried at least one of the urovirulence sequences. The molecular typing system revealed a pathotype\u2010specific clonal group distribution and showed a closer relationship between the EAEC and UPEC. Additionally, the occurrence of urovirulence traits, especially those related to iron acquisition, was more frequent among EAEC and UPEC than among the other <jats:italic>E. coli<\/jats:italic> pathotypes. This observation is of special value considering that the EAEC pathotype constitutes an emerging group of enteropathogens, particularly, in developing countries, and information on their pathogenic and phylogenetic characteristics is still scarce. (\u00a9 2010 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jobm.200900364","type":"journal-article","created":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T14:52:44Z","timestamp":1283266364000},"source":"Crossref","is-referenced-by-count":20,"title":["Molecular characterization of uropathogenic and diarrheagenic <i>Escherichia coli<\/i> pathotypes"],"prefix":"10.1002","volume":"50","author":[{"given":"Adriana Hamond","family":"Regua\u2010Mangia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinue","family":"Irino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"da Silva Pacheco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rose Mary","family":"Pimentel Bezerra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 Reynaldo","family":"Santos P\u00e9riss\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia Martins","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,8,30]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.06.008"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-007-0506-Y"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-9-88"},{"key":"e_1_2_1_5_2","first-page":"1085","volume":"21","author":"Escobar\u2010P\u00e1ramo P.","journal-title":"2004. 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