{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:42:57Z","timestamp":1780497777514,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2010,8,30]],"date-time":"2010-08-30T00:00:00Z","timestamp":1283126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>IceA<\/jats:italic> of <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>) has been suggested as a virulence factor for the bacteria, but its pathogenic role remains unelucidated. Here, we examined the effect of <jats:italic>iceA<\/jats:italic> mutation on the secretion of IL\u20108 by human gastric epithelial cells. We also investigated whether the changes in IL\u20108 production caused by <jats:italic>iceA<\/jats:italic> mutation were associated with impaired adherence of <jats:italic>H. pylori<\/jats:italic> to the epithelial cells or with impaired apoptosis of these cells. The <jats:italic>iceA<\/jats:italic> mutant strain was constructed from wildtype <jats:italic>H. pylori<\/jats:italic> strain by insertional mutagenesis of <jats:italic>iceA<\/jats:italic>. The human gastric epithelial cells SGC7901 were infected with wildtype or mutant <jats:italic>H. pylori<\/jats:italic> for appropriate lengths of time. The adherence of the bacteria to the epithelial cells was examined by fluorescent microscopy using an anti\u2010<jats:italic>H. pylori<\/jats:italic> antibody and flow cytometry. The apoptosis of the epithelial cells was studied by annexin\u2010V staining and flow cytometry. The production of IL\u20108 by SGC 7901 cells was determined by ELISA. We found that <jats:italic>iceA<\/jats:italic> mutation was associated with significantly impaired production of IL\u20108 from the epithelial cells, which was not due to impaired adherence by the bacteria to the epithelial cells as wildtype and mutant <jats:italic>H. pylori<\/jats:italic> exhibited similar levels of binding to the epithelial cells. Furthermore, inactivation of <jats:italic>iceA<\/jats:italic> did not affect the apoptotic cell death of SGC7901. Our findings indicate that <jats:italic>iceA<\/jats:italic> may contribute to the pathogenicity of <jats:italic>H. pylori<\/jats:italic> by modulating the production of IL\u20108 by host epithelial cells. (\u00a9 2010 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jobm.200900410","type":"journal-article","created":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T14:52:36Z","timestamp":1283266356000},"source":"Crossref","is-referenced-by-count":14,"title":["Mutation of <i>iceA<\/i> in <i>Helicobacter pylori<\/i> compromised IL\u20108 induction from human gastric epithelial cells"],"prefix":"10.1002","volume":"50","author":[{"given":"Yi Jun","family":"Ma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang Cai","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong Guang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing Tang","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei Dong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,8,30]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.720"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199104113241507"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-007-9803-7"},{"key":"e_1_2_1_5_2","first-page":"531","article-title":"Adherence to gastric epithelial cells induces expression of a Helicobacter pylori gene, iceA, that is associated with clinical outcome","volume":"110","author":"Peek R.M.","year":"1998","journal-title":"Proc. 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