{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:07:21Z","timestamp":1791205641817,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,2,7]],"date-time":"2011-02-07T00:00:00Z","timestamp":1297036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aerobic methylotrophic bacteria able to grow with dichloromethane (DCM) as the sole carbon and energy source possess a specific glutathione S\u2010transferase, DCM dehalogenase, which transforms DCM to formaldehyde, used for biomass and energy production, and hydrochloric acid, which is excreted. Evidence is presented for chloride\u2010specific responses for three DCM\u2010degrading bacteria, <jats:italic>Methylobacterium extorquens<\/jats:italic> DM4, <jats:italic>Methylopila helvetica<\/jats:italic> DM6 and <jats:italic>Albibacter methylovorans<\/jats:italic> DM10. Chloride release into the medium was inhibited by sodium azide and <jats:italic>m<\/jats:italic> \u2010chlorophenylhydrazone, suggesting an energy\u2010dependent process. In contrast, only nigericin affected chloride excretion in <jats:italic>Mb. extorquens<\/jats:italic> DM4 and <jats:italic>Mp. helvetica<\/jats:italic> DM6, while valinomycin had the same effect in <jats:italic>A. methylovorans<\/jats:italic> DM10 only. Chloride ions stimulated DCM\u2010dependent induction of DCM dehalogenase expression for <jats:italic>Mp. helvetica<\/jats:italic> DM6 and <jats:italic>A. methylovorans<\/jats:italic> DM10, and shortened the time for onset of chloride release into the medium. Striking chloride\u2010containing structures were observed by electron microscopy and X\u2010ray microanalysis on the cell surface of <jats:italic>Mp. helvetica<\/jats:italic> DM6 and <jats:italic>A. methylovorans<\/jats:italic> DM10 during growth with DCM, and with methanol in medium supplemented with sodium chloride. Taken together, these data suggest the existence of both general and specific chloride\u2010associated adaptations in aerobic DCM\u2010degrading bacteria. (\u00a9 2011 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jobm.201000280","type":"journal-article","created":{"date-parts":[[2011,2,7]],"date-time":"2011-02-07T08:21:25Z","timestamp":1297066885000},"page":"296-303","source":"Crossref","is-referenced-by-count":22,"title":["Chloride\u2010associated adaptive response in aerobic methylotrophic dichloromethane\u2010utilising bacteria"],"prefix":"10.1002","volume":"51","author":[{"given":"Maria L.","family":"Torgonskaya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina V.","family":"Doronina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith","family":"Hourcade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri A.","family":"Trotsenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Vuilleumier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,2,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.23.13641"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(00)80007-7"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-3-1051"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-11-2967"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2009.02.002"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10438-005-0086-5"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","unstructured":"Imhoff J.F. 1993.Osmotic adaptation in halophilic and halotolerant microorganisms. 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