{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:30:52Z","timestamp":1792182652080,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,11,3]],"date-time":"2011-11-03T00:00:00Z","timestamp":1320278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Biosurfactants of rhamnolipids have been enthusiastically investigated for substitutes of synthetic agrochemicals against plant pathogens. However, all such studies have been conducted on rhamnolipids with high purity which have limitations due to high costs. This paper focused on the applicability of rhamnolipid\u2010containing cell\u2010free culture broth. It was found that rhamnolipids in cell\u2010free culture broth of\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ZJU211 were largely composed of di\u2010rhamnolipid and mono\u2010rhamnolipid with the ratio varying with culture time. After 96 h of fermentation, the mass ratio of di\u2010rhamnolipid over mono\u2010rhamnolipid increased to 2.6:1. Crude rhamnolipids in a form of cell\u2010free culture broth showed high antifungal activity against colony growth and biomass accumulation of seven plant pathogens comprising two\n                    <jats:italic>Oomycetes<\/jats:italic>\n                    , three\n                    <jats:italic>Ascomycota<\/jats:italic>\n                    and two\n                    <jats:italic>Mucor<\/jats:italic>\n                    spp. fungi, among which three plant pathogens were firstly reported in this paper showing inhibition to rhamnolipids. Particularly, rhamnolipids showed potent activity against two\n                    <jats:italic>Oomycetes<\/jats:italic>\n                    that acquire resistance to commercial compound of metalaxyal. Furthermore, di\u2010rhamnolipid was elucidated to dominate the antifungal activity of crude rhamnolipids by\n                    <jats:italic>in vitro<\/jats:italic>\n                    studies. At last, the efficacy and safety of cell\u2010free culture broth was preliminarily illustrated on plants\n                    <jats:italic>in vivo<\/jats:italic>\n                    . So cell\u2010free culture broth as a crude rhamnolipid product could be served as a potential cost\u2010effective and environmental\u2010friendly fungicide in agriculture. (\u00a9 2012 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)\n                  <\/jats:p>","DOI":"10.1002\/jobm.201100295","type":"journal-article","created":{"date-parts":[[2011,11,3]],"date-time":"2011-11-03T09:12:06Z","timestamp":1320311526000},"page":"458-466","source":"Crossref","is-referenced-by-count":105,"title":["Producing cell\u2010free culture broth of rhamnolipids as a cost\u2010effective fungicide against plant pathogens"],"prefix":"10.1002","volume":"52","author":[{"given":"Ruyi","family":"Sha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lifang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhirong","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,11,3]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01334-07"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2009.10.017"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-010-9725-z"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04513.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10474"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2010.06.026"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0290(20010105)72:1<25::AID-BIT4>3.0.CO;2-J"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2007.03701.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2007.02.026"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:ANTO.0000020148.45523.41"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf104027x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS.1997.81.1.4"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/1526-4998(200012)56:12<1029::AID-PS238>3.0.CO;2-Q"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3059.2005.01140.x"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2007.01501.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-007-1828-4"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2006.09.005"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2000.00961.x"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2008.01911.x"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2498-2"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-006-9187-4"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jim.2900450"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.2007.A1514"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2008.11.003"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0260-8774(01)00214-X"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-009-8707-8"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS.2001.85.10.1069"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2010.02858.x"},{"key":"e_1_2_1_30_2","first-page":"3187","article-title":"Effects of Validamycin A on the morphology, growth and sporulation of Rhizoctonia cerealis, Fusarium culmorum and other fungi","volume":"134","author":"Geoffrey D.R.","year":"1988","journal-title":"J. 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