{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:50:31Z","timestamp":1775152231283,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,5,17]],"date-time":"2013-05-17T00:00:00Z","timestamp":1368748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The rapid and widespread dissemination of CTX\u2010M\u2010type extended\u2010spectrum beta\u2010lactamases (ESBLs) <jats:italic>Escherichia coli<\/jats:italic> in most of the provinces in China has been previously characterized; however, these enzymes have been rarely reported among minority regions. A prospective study using 180 <jats:italic>E. coli<\/jats:italic> clones from three hospitals in the minority district of Dali Bai was conducted between January 2009 and June 2010 to characterize the profiles of <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M<\/jats:sub>\u2010producing <jats:italic>E. coli<\/jats:italic>. Overall, 87 of the isolates (48.3%) encoded the resistance phenotypes of ESBLs. Among these 87 isolates, 56 (64.4%) exconjugants were successfully obtained. The genotypes of <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M<\/jats:sub> were evaluated by PCR and DNA sequencing. In total, 22 and 53 <jats:italic>E. coli<\/jats:italic> isolates possessed CTX\u2010M cluster 1 and CTX\u2010M cluster 9, respectively. Five <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M<\/jats:sub> subtypes were detected, namely, <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M3<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u200912), <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M15<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u20094), <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M22<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u20092), <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M14<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u200941), and <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M9<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u20098). The broth microdilution method was performed to determine the minimal inhibitory concentration of 13 antibiotics. Most CTX\u2010M producers were multiply resistant to both \u03b2\u2010lactam and non\u2010\u03b2\u2010lactam antibiotics. Imipenem showed excellent activity, and only one isolate harboring <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M14+CTX\u2010M3<\/jats:sub> exhibited resistance to it. In conclusion, there seems to be great concern regarding the distribution and drug resistance of CTX\u2010M ESBL\u2010producing <jats:italic>E. coli<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jobm.201200313","type":"journal-article","created":{"date-parts":[[2013,5,17]],"date-time":"2013-05-17T11:57:58Z","timestamp":1368791878000},"page":"247-252","source":"Crossref","is-referenced-by-count":8,"title":["Characterization of CTX\u2010M\u2010type extended\u2010spectrum beta\u2010lactamase in clinical clones of <i>Escherichia coli<\/i> in Southwest China"],"prefix":"10.1002","volume":"54","author":[{"given":"Wei\u2010Dong","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Clinical Medicine Dali University Dali Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The Fourth Hospital of Yunnan Province Dali Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai\u2010Ning","family":"Guan","sequence":"additional","affiliation":[{"name":"Institute of Pathogens and Vectors, Faculty of Basic Medicine Dali University Dali Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qun\u2010Zhi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Clinical Medicine Dali University Dali Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,5,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00576-07"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.4.1249-1255.2004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01650718"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl483"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.004507-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp068"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn164"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn182"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01446-10"},{"key":"e_1_2_7_11_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing, 19th Informational Supplement M100\u2013S19","author":"CLSI","year":"2009"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5715-5721.2004"},{"key":"e_1_2_7_13_1","first-page":"325","article-title":"CHINET 2009 surveillance of bacterial resistance in China","volume":"5","author":"Wang F.","year":"2010","journal-title":"Chin. 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