{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:01:26Z","timestamp":1791313286404,"version":"4.3.1"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,5,26]],"date-time":"2013-05-26T00:00:00Z","timestamp":1369526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:sec><jats:label\/><jats:p>The effect of EGTA on the adhesion and on the formation of a biofilm by two reference and eight clinical strains of <jats:italic>Staphylococcus aureus<\/jats:italic> was studied. All the clinical strains were isolated from patients from Kinshasa. <jats:italic>Spa<\/jats:italic> typing confirmed that these clinical strains were distinct. The Biofilm Ring Test (BFRT\u00ae) showed that EGTA (100\u2009\u00b5M\u201310\u2009mM) inhibited the adhesion of the four clinical methicillin\u2010resistant (MRSA) strains and the crystal violet staining method that it inhibited the formation of a biofilm by all the strains. Divalent cations abolished the effect of EGTA on the formation of a biofilm, specially in the clinical MRSA strains. EGTA had no effect on established biofilms. Only concentrations of EGTA higher than 10\u2009mM were toxic to eukaryotic cells. Our results establish the effectiveness and the safety of lock solutions with EGTA to prevent the formation <jats:italic>in vitro<\/jats:italic> of biofilms by <jats:italic>S. aureus<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jobm.201200511","type":"journal-article","created":{"date-parts":[[2013,5,26]],"date-time":"2013-05-26T11:25:05Z","timestamp":1369567505000},"page":"700-710","source":"Crossref","is-referenced-by-count":5,"title":["Inhibition by <scp>EGTA<\/scp> of the formation of a biofilm by clinical strains of <i>Staphylococcus aureus<\/i>"],"prefix":"10.1002","volume":"54","author":[{"given":"J. M.","family":"Liesse Iyamba","sequence":"first","affiliation":[{"name":"Laboratoire de Chimie Biologique et M\u00e9dicale et de Microbiologie Pharmaceutique Facult\u00e9 de Pharmacie, Universit\u00e9 Libre de Bruxelles Brussels Belgium"},{"name":"Laboratoire de Microbiologie Exp\u00e9rimentale et Pharmaceutique Facult\u00e9 des Sciences Pharmaceutiques, Universit\u00e9 de Kinshasa Kinshasa Democratic Republic of Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Seil","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Biologique et M\u00e9dicale et de Microbiologie Pharmaceutique Facult\u00e9 de Pharmacie, Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Nagant","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Biologique et M\u00e9dicale et de Microbiologie Pharmaceutique Facult\u00e9 de Pharmacie, Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Dulanto","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Biologique et M\u00e9dicale et de Microbiologie Pharmaceutique Facult\u00e9 de Pharmacie, Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Deplano","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence<i>S. aureus<\/i> Laboratoire de Microbiologie, H\u00f4pital Erasme Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"El Khattabi","sequence":"additional","affiliation":[{"name":"Laboratoire de Physiologie et de Pharmacologie Facult\u00e9 de Pharmacie, Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. B.","family":"Takaisi Kikuni","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence<i>S. aureus<\/i> Laboratoire de Microbiologie, H\u00f4pital Erasme Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. P.","family":"Dehaye","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Biologique et M\u00e9dicale et de Microbiologie Pharmaceutique Facult\u00e9 de Pharmacie, Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1811-1819.2005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/317606"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.2.167-193.2002"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.clpt.6100247"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.1.175-183.1996"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1128\/JCM.37.10.3235-3238.1999","article-title":"Slime production and expression of slime\u2010associated antigen by staphylococcal clinical isolates","volume":"37","author":"Ammendolia M.G.","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.5427-5433.1999"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00688.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-75418-3_10"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01913-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.11.010"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.112408.134306"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2011.604776"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.11.010"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(97)00463-2"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.7.1685-1691.1992"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.4.1514-1517.2002"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01866-06"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.2.619-621.2006"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2010.06.011"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1311"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2004.12.011"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007567"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26412-0"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.2.620-630.2002"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.200700289"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4465-4467.2003"},{"key":"e_1_2_7_29_1","doi-asserted-by":"crossref","first-page":"4351","DOI":"10.1128\/JCM.38.12.4351-4355.2000","article-title":"Glyceraldehyde\u20103\u2010phosphate dehydrogenase\u2010encoding gene as a useful taxonomic tool for Staphylococcus spp","volume":"38","author":"Yugueros J.","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_7_30_1","first-page":"63","article-title":"Simultaneous PCR detection of ica cluster and methicillin and mupirocin resistance genes in catheter\u2010isolated Staphylococcus","volume":"7","author":"Mart\u00edn\u2010Lopez J.","year":"2004","journal-title":"Int. Microbiol."},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfl170"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1479050504001140"},{"key":"e_1_2_7_33_1","first-page":"143","article-title":"Biomaterial specifity, molecular mechanisms and clinical relevance of S. epidermidis and S. aureus infections in surgery","volume":"16","author":"Gristina A.G.","year":"1987","journal-title":"Zbl. Bakt."},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1992.tb02106.x"},{"key":"e_1_2_7_35_1","unstructured":"Smith R.M. Martell A.E. Motekaitis R.J. 2001.NIST Critically selected stability constants of metal complexes database. Version 6.0. NIST Standard Reference Database Vol. 46 National Institute of Standards and Technology US Department of Commerce Gaithersburg (MD)."},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2000.tb01496.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01627256"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1067-1927.2004.abstractbg.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1088\/1742-6596\/252\/1\/012014"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.040758-0"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/006676-0"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01947-07"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03534.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.06.005"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-92"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47757-0"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03367.x"}],"container-title":["Journal of Basic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjobm.201200511","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jobm.201200511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T20:49:47Z","timestamp":1694551787000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jobm.201200511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,26]]},"references-count":46,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1002\/jobm.201200511"],"URL":"https:\/\/doi.org\/10.1002\/jobm.201200511","archive":["Portico"],"relation":{},"ISSN":["0233-111X","1521-4028"],"issn-type":[{"value":"0233-111X","type":"print"},{"value":"1521-4028","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,26]]}}}