{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T18:04:24Z","timestamp":1792260264116,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,5,20]],"date-time":"2013-05-20T00:00:00Z","timestamp":1369008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Plant protection through siderophore producing rhizobacteria (SPR) has emerged as a sustainable approach for crop health management. In present study, 220 bacteria isolated from tomato rhizosphere were screened for <jats:italic>in vitro<\/jats:italic> antagonistic activity against <jats:italic>Rhizoctonia solani<\/jats:italic> AG\u20104. Nine potent antagonistic strains viz., <jats:italic>Alcaligenes<\/jats:italic> sp. (MUN1, MB21, and MPF37), <jats:italic>Enterobacter<\/jats:italic> sp. (MPM1), <jats:italic>Pseudomonas<\/jats:italic> sp. (M10A and MB65), <jats:italic>P. aeruginosa<\/jats:italic> (MPF14 and MB123) and <jats:italic>P. fluorescens<\/jats:italic> (MPF47) were identified on the basis of physiological characters and 16S rDNA sequencing. These strains were able to produce hydrolytic enzymes, hydrogen cyanide, indole acetic acid, although, only few strains were able to solubilize phosphate. Two strains (MB123 and MPF47) showed significant disease reduction in glasshouse conditions were further evaluated under field conditions using three different application methods. Application of <jats:italic>P. fluorescens<\/jats:italic> (MPF47) in nursery as soil mix\u2009+\u2009seedling root treatments prior to transplantation resulted in significant disease reduction compared to control. Total chlorophyll and available iron were significantly higher in the MPF47 treated plants in contrast to infected control. In conclusion, siderophore producing bacteria MPF47 have strong biocontrol abilities and its application as soil mix\u2009+\u2009seedling root treatments provided strong shield to plant roots against <jats:italic>R. solani<\/jats:italic> and could be used for effective bio\u2010management of pathogen.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jobm.201200564","type":"journal-article","created":{"date-parts":[[2013,5,20]],"date-time":"2013-05-20T09:34:44Z","timestamp":1369042484000},"page":"585-597","source":"Crossref","is-referenced-by-count":75,"title":["Isolation and characterization of siderophore producing antagonistic rhizobacteria against <i>Rhizoctonia solani<\/i>"],"prefix":"10.1002","volume":"54","author":[{"given":"Manoj Kumar","family":"Solanki","sequence":"first","affiliation":[{"name":"National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supriya","family":"Srivastava","sequence":"additional","affiliation":[{"name":"National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudheer","family":"Kumar","sequence":"additional","affiliation":[{"name":"National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prem Lal","family":"Kashyap","sequence":"additional","affiliation":[{"name":"National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alok K.","family":"Srivastava","sequence":"additional","affiliation":[{"name":"National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilip K.","family":"Arora","sequence":"additional","affiliation":[{"name":"National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,20]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"NBH 2010. Indian horticulture database National Board of Horticulture India."},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-007-9505-5"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/09583157.2011.649713"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12600-011-0188-y"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2011.03.006"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-010-9740-0"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-008-9264-z"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1129"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2008.10.003"},{"key":"e_1_2_5_11_1","first-page":"925","article-title":"Plant growth promoting potential of P solubilizing Pseudomonas sp. occuring in acidic soil of Jalgaon","volume":"4","author":"Sayyed R.Z.","year":"2007","journal-title":"Asian J. Microbiol. Biotechnol. Environ. Sci."},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejsobi.2010.11.001"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-97-2-0250"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-012-1003-8"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-7-0440"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/09583159309355278"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008747926678"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020557523557"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002039900127"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1094\/PD-79-0782"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0261-2194(96)00045-2"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00369386"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0003-2697(87)90612-9","article-title":"Universal chemical assay for the detection and determination of siderophores","volume":"160","author":"Schwyn B.","year":"1987","journal-title":"Ann. Biochem."},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1139\/m69-122"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0885-5765(91)90029-H"},{"key":"e_1_2_5_26_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"139","author":"Lowry O.","year":"1951","journal-title":"J. Biol. Chem."},{"key":"e_1_2_5_27_1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1128\/aem.57.2.535-538.1991","article-title":"Rapid in situ assay for indole acetic acid production by bacteria immobilized on a nitrocellulose membrane","volume":"57","author":"Bric J.M.","year":"1991","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_2_5_28_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.21082\/ijas.v4n1.2003.p27-31","article-title":"Screening of soil bacteria for plant growth promotion activities in vitro","volume":"4","author":"Husen E.","year":"2003","journal-title":"Indian J. Agric. Sci."},{"key":"e_1_2_5_29_1","first-page":"125","volume-title":"Microbiology, A Laboratory Manual","author":"Cappuccino J.C.","year":"1992"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1948.tb07118.x"},{"key":"e_1_2_5_31_1","first-page":"93","volume-title":"Bergey's Manual of Determinative Bacteriology","author":"Holt J.G.","year":"1994"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-012-0160-1"},{"key":"e_1_2_5_33_1","first-page":"1","article-title":"The water culture method for growing plants without soil","volume":"347","author":"Hoagland D.R.","year":"1950","journal-title":"Calif. Agric. Exp. Sta. Circ."},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.102.017277"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02149714"},{"key":"e_1_2_5_36_1","first-page":"187","article-title":"Siderophores in relation to plant growth and disease","volume":"37","author":"Neilands J.B.","year":"1986","journal-title":"Ann. Rev. Phytopathol."},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/jr9540002588"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)74509-2"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/01904169209364466"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-8524(00)00064-X"},{"key":"e_1_2_5_41_1","first-page":"1","article-title":"Plant growth promoting rhizobacteria: a critical review","volume":"21","author":"Saharan B.S.","year":"2011","journal-title":"Life Sci. Med. Res."},{"key":"e_1_2_5_42_1","first-page":"109","article-title":"In vitro suppression of plant pathogens by siderophores of fluorescent pseudomonads","volume":"40","author":"Manwar A.V.","year":"2000","journal-title":"Ind. J. Microbiol."},{"key":"e_1_2_5_43_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1128\/aem.63.1.99-105.1997","article-title":"Availability of iron to Pseudomonas fluorescence in rhizosphere and bulk soil evaluated with an ice nucleation reporter gene","volume":"63","author":"Loper J.E.","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_2_5_44_1","doi-asserted-by":"crossref","first-page":"3563","DOI":"10.1128\/AEM.64.10.3563-3569.1998","article-title":"Secondary metabolite\u2010 and endochitinase\u2010dependent antagonism toward plant\u2010pathogenic microfungi of Pseudomonas fluorescens isolates from sugar beet rhizosphere","volume":"64","author":"Nielsen M.N.","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2004.01.005"},{"key":"e_1_2_5_46_1","first-page":"1578","article-title":"Beneficial effects of fluorescent Pseudomonads on seed germination, growth promotion, and suppression of charcoal rot in groundnut (Arachis hypogea L.)","volume":"18","author":"Bhatia S.","year":"2008","journal-title":"J. Microbiol. Biotechnol."},{"key":"e_1_2_5_47_1","first-page":"207","article-title":"In\u2010vitro antagonistic potential of fluorescent Pseudomonads and control of sheath blight of maize caused by Rhizoctonia solani","volume":"42","author":"Tripathi M.","year":"2002","journal-title":"Indian J. Microbiol."},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/03235408.2010.482419"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1094\/PD-74-0812"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1080\/01904168609363464"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-007-9167-1"}],"container-title":["Journal of Basic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjobm.201200564","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jobm.201200564","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T20:50:13Z","timestamp":1694551813000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jobm.201200564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,20]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1002\/jobm.201200564"],"URL":"https:\/\/doi.org\/10.1002\/jobm.201200564","archive":["Portico"],"relation":{},"ISSN":["0233-111X","1521-4028"],"issn-type":[{"value":"0233-111X","type":"print"},{"value":"1521-4028","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,20]]}}}