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Extraction and analysis of proteins induced by heavy metals can help to understand the regulatory mechanisms of <jats:italic>P. chrysosporium<\/jats:italic> in wastewater treatment. In this study, <jats:italic>P. chrysosporium<\/jats:italic> was exposed to 50\u2009\u03bcM cadmium nitrate. A maximum cadmium adsorption capability of 77.1\u2009mg\/g dry biomass was found after 65\u2009h, which was accompanied by a relatively higher protein concentration. After separation of the culture fluid proteins by two\u2010dimensional difference gel electrophoresis (2D\u2010DIGE), three differentially expressed proteins were detected from 17 spots. By using 2D\u2010DIGE, followed by matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight\/time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF\/TOF MS), glutathione <jats:italic>S<\/jats:italic>\u2010transferase, glyceraldehyde\u20103\u2010phosphate dehydrogenase, and malate dehydrogenase were identified. 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