{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T09:28:45Z","timestamp":1775208525822,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,1,30]],"date-time":"2015-01-30T00:00:00Z","timestamp":1422576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:sec><jats:label\/><jats:p>To ascertain the variability in <jats:italic>Fusarium udum<\/jats:italic> (<jats:italic>Fu<\/jats:italic>) isolates associated with pigeonpea wilt is a difficult task, if based solely on morphological and cultural characters. In this respect, the robustness of five different genetic marker <jats:italic>viz<\/jats:italic>., random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus (ERIC), BOX elements, mating type locus, and microsatellite markers were employed to decipher intra\u2010specific variability in <jats:italic>Fu<\/jats:italic> isolates. All techniques yielded intra\u2010specific polymorphism, but different levels of discrimination were obtained. RAPD\u2010PCR was more discriminatory, enabling the detection of thirteen variants among twenty <jats:italic>Fu<\/jats:italic> isolates. By microsatellite, ERIC\u2010 and BOX\u2010PCR fingerprinting, the isolates were categorized in seven, five, and two clusters, respectively. Cluster analysis of the combined data also showed that the <jats:italic>Fu<\/jats:italic> isolates were grouped into ten clusters, sharing 50\u2013100% similarity. The occurrence of both mating types in <jats:italic>Fu<\/jats:italic> isolates is reported for the first time in this study. All examined isolates harbored one of the two mating\u2010type idiomorphs, but never both, which suggests a heterothallic mating system of sexual reproduction among them. Information obtained from comparing results of different molecular marker systems should be useful to organize the genetic variability and ideally, will improve disease management practices by identifying sources of inoculum and isolate characteristics.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jobm.201400483","type":"journal-article","created":{"date-parts":[[2015,1,30]],"date-time":"2015-01-30T10:50:45Z","timestamp":1422615045000},"page":"846-856","source":"Crossref","is-referenced-by-count":27,"title":["Mating type genes and genetic markers to decipher intraspecific variability among <i>Fusarium udum<\/i> isolates from pigeonpea"],"prefix":"10.1002","volume":"55","author":[{"given":"Prem Lal","family":"Kashyap","sequence":"first","affiliation":[{"name":"ICAR\u2010National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shalini","family":"Rai","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudheer","family":"Kumar","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"},{"name":"ICAR\u2010 Directorate of Wheat Research Karnal Haryana 132001 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alok Kumar","family":"Srivastava","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Anandaraj","sequence":"additional","affiliation":[{"name":"ICAR\u2010Indian Institute of Spices Research (IISR) Marikunnu Calicut Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arun Kumar","family":"Sharma","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Agriculturally Important Microorganisms Mau Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,1,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-10-45"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12600-013-0324-y"},{"key":"e_1_2_6_4_1","first-page":"163","article-title":"Genetic variability among Fusarium udum isolates from pigeonpea","volume":"13","author":"Kiprop E.K.","year":"2005","journal-title":"Afr. 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