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Basic Microbiol."],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:label\/><jats:p>Sco7697, a gene encoding a phytase, enzyme able to degrade phytate (myo\u2010inositol 1,2,3,4,5,6\u2010hexakis phosphate), the most abundant phosphorus storing compound in plants is present in the genome of <jats:italic>S. coelicolor<\/jats:italic>, a soil born bacteria with a saprophytic lifestyle. The expression of this gene was previously shown to be induced in conditions of Pi limitation by the response regulator PhoP binding to an operator sequence, the PHO box, located upstream of the \u221235 promoter sequence. A close examination of the promoter region of sco7697 revealed the presence of another putative operator site, a Direct Repeat (DR), located downstream of the \u221210 promoter sequence. In order to determine whether this DR played a role in regulation of sco7697 expression, different variants of the phytase gene promoter region were transcriptionally fused to the \u00df\u2010glucuronidase reporter gene (GUS). As expected, deletion of the PHO box led to abolition of sco7697 induction in conditions of Pi limitation. Interestingly, alteration of the DR correlated with a dramatic increase of GUS expression but only when PhoP was present. These results demonstrated that this DR is the site of strong negative regulation by an unknown repressor. The latter would impede the necessary activation of phytase expression by PhoP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jobm.201500417","type":"journal-article","created":{"date-parts":[[2015,9,11]],"date-time":"2015-09-11T12:06:11Z","timestamp":1441973171000},"page":"59-66","source":"Crossref","is-referenced-by-count":10,"title":["Evidence for the negative regulation of phytase gene expression in <i>Streptomyces lividans<\/i> and <i>Streptomyces coelicolor<\/i>"],"prefix":"10.1002","volume":"56","author":[{"given":"Ines","family":"Boukhris","sequence":"first","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS University Paris\u2010Sud Orsay France"},{"name":"Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax University of Sfax Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Dulermo","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS University Paris\u2010Sud Orsay France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hichem","family":"Chouayekh","sequence":"additional","affiliation":[{"name":"Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax University of Sfax Sfax Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Jo\u00eblle","family":"Virolle","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS University Paris\u2010Sud Orsay France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2164(00)47004-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-7-2097"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-003-1530-1"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf00109a001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0271-5317(95)00040-P"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2621.2002.00618.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2004.03.010"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2109.2005.01290.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0518554"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2013-03465"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2006.06.010"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi1010249"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-0929.2008.00590.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-0929.2009.00631.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2008-00043"},{"key":"e_1_2_7_17_1","first-page":"1425","article-title":"Molecular and biochemical characteristics of \u03b2\u2010propellar phytase from marine Pseudomonas sp. 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