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These NPs were characterized by Ultra violet visible spectroscopy, Fourier Transform Infra Red, Atomic Force Microscopy, and Transmission Electron Microscopy (TEM). The average particle size of green synthesized CN\u2010AgNPs was 15.2\u2009\u00b1\u20091.01\u2009nm. Gas chromatography\u2010 mass spectrometry analysis of methanolic bark extract involved in the formation of CN\u2010AgNPs revealed lupeol as a major active component. In this study, CN\u2010AgNPs (15\u2009\u03bcg\u2009ml<jats:sup>\u22121<\/jats:sup>) efficiently suppressed the production of quorum sensing mediated virulence factors viz. pyocyanin, protease, hemolysin, and biofilm formation in<jats:italic>Pseudomonas aeruginosa<\/jats:italic>. The pyocyanin production was strongly inhibited (74.64%) followed by proteolysis (47.3%) and hemolysin production (47.7%). However, the biofilm forming ability was maximally reduced up to 79.70%. Moreover, the Confocal Laser Scanning Microscopic Analysis showed that CN\u2010AgNPs inhibit colonization of<jats:italic>P. aeruginosa<\/jats:italic>on to the surface. Furthermore, TEM analysis revealed internalization of CN\u2010AgNPs inside the bacterial cell. It is concluded that green synthesized AgNPs have great potential to inhibit virulence factors and biofilm forming ability of drug\u2010resistant clinical isolates of<jats:italic>P<\/jats:italic>.<jats:italic>aeruginosa<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jobm.201600175","type":"journal-article","created":{"date-parts":[[2016,11,22]],"date-time":"2016-11-22T12:37:35Z","timestamp":1479818255000},"page":"193-203","source":"Crossref","is-referenced-by-count":43,"title":["<i>Crataeva nurvala<\/i>nanoparticles inhibit virulence factors and biofilm formation in clinical isolates of<i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1002","volume":"57","author":[{"given":"Syed Ghazanfar","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Microbiology Nanotechnology and Antimicrobial Drug Resistance Research Laboratory Jawaharlal Nehru Medical College and Hospital Aligarh Muslim University Aligarh Uttar Pradesh 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